Anti-Human TNFR2/CD120b Rabbit Recombinant Antibody, PBS Only
TNFR2/CD120b Recombinant Antibody for FC, Cytometric bead array, Indirect ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
FC, Cytometric bead array, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
230328B3
Cat no : 98010-1-PBS
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
98010-1-PBS targets TNFR2/CD120b as part of a matched antibody pair:
MP00777-1: 83101-2-PBS capture and 98010-1-PBS detection (validated in Cytometric bead array)
MP00777-2: 83101-3-PBS capture and 98010-1-PBS detection (validated in Cytometric bead array)
MP00777-3: 83101-4-PBS capture and 98010-1-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | tumor necrosis factor receptor superfamily, member 1B |
| Calculated molecular weight | 48 kDa |
| GenBank accession number | BC052977 |
| Gene symbol | TNFR2 |
| Gene ID (NCBI) | 7133 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS only |
| Storage Conditions | Store at -80°C. Stable for one year after shipment. |
Background Information
Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for TNFR2/CD120b antibody 98010-1-PBS | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
