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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNFRSF6B Polyclonal antibody (24999-1-AP)

    WB analysis using 24999-1-AP

    SW480 cells were subjected to SDS PAGE followed by western blot with 24999-1-AP (TNFRSF6B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using TNFRSF6B Polyclonal antibody (24999-1-AP)

    IP experiment of HeLa using 24999-1-AP

    IP result of anti-TNFRSF6B (IP:24999-1-AP, 4ug; Detection:24999-1-AP 1:300) with HeLa cells lysate 2480ug.

  • Flow cytometry (FC) experiment of SW480 cells using TNFRSF6B Polyclonal antibody (24999-1-AP)

    FC experiment of SW480 using 24999-1-AP

    1x10^6 SW480 cells were intracellularly stained with 0.25 ug TNFRSF6B Polyclonal antibody (24999-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2) (red) or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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TNFRSF6B Polyclonal antibody

TNFRSF6B Polyclonal Antibody for WB, FC (Intra), IP, ELISA
Cat No. 24999-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), IP, ELISA

Synonyms

UNQ186/PRO212, TR6, M68, Decoy receptor for Fas ligand, Decoy receptor 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSW480 cells
Positive IP detected inHeLa cells
Positive FC (Intra) detected inSW480 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

24999-1-AP targets TNFRSF6B in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNFRSF6B fusion protein Ag19781 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 6b, decoy
Calculated molecular weight 300 aa, 33 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC017065
Gene Symbol TNFRSF6B
Gene ID (NCBI) 8771
RRIDAB_2879838
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95407
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TNFRSF6B antibody 24999-1-APDownload protocol
IP protocol for TNFRSF6B antibody 24999-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation

Authors - Nasha Zhang
View Article