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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibody

TNFRSF9/CD137 Recombinant Antibody for IF-P, FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF-P, FC

Conjugate

Unconjugated

CloneNo.

241344F3

Cat no : 98112-1-RR

Synonyms

TNFRSF9, ILA, CD137, 4-1BB ligand receptor, 4 1BB ligand receptor

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibo (98112-1-RR)

    IF Staining of human tonsillitis using 98112-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using TNFRSF9/CD137 antibody (98112-1-RR, Clone: 241344F3 ) at dilution of 1:180 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibo (98112-1-RR)

    IF Staining of human tonsillitis using 98112-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using TNFRSF9/CD137 antibody (98112-1-RR, Clone: 241344F3 ) at dilution of 1:180 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibo (98112-1-RR)

    FC experiment of human PBMCs using 98112-1-RR

    1x10^6 unstimulated or Con-A-stimulated human PBMCs were surface stained with 0.25 ug Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibody (98112-1-RR, Clone: 241344F3) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug APC Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (APC-65559, Clone: UCHT4). Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibo (98112-1-RR)

    FC experiment of human PBMCs using 98112-1-RR

    1x10^6 unstimulated (black) or Con-A-stimulated human PBMCs (red) were surface stained with 0.25 ug Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibody (98112-1-RR, Clone: 241344F3) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), or Isotype Control (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98112-1-RR

    Affinity and Kinetic Characterization of 98112-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98112-1-RR against Human TNFRSF9/CD137 were performed. The affinity constant is 10.3 nM.

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Tested Applications

Positive IF-P detected inhuman tonsillitis tissue
Positive FC detected inCon-A-stimulated human PBMCs

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98112-1-RR targets TNFRSF9/CD137 in IF-P, FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 9
Calculated molecular weight 28 kDa
GenBank accession numberBC006196
Gene symbol TNFRSF9/CD137
Gene ID (NCBI) 3604
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD137, also known as TNFRSF9 or 4-1BB, is an inducible T cell surface receptor that belongs to the tumor necrosis factor receptor superfamily. CD137 is a transmembrane protein expressed on the surface of activated T-cells. In addition, activation-dependent expression of CD137 has also been found in B lymphocytes, monocytes, and diverse nonlymphoid cell types. CD137 provides a co-stimulatory signal that enhances the survival, and differentiation of cells, and has a crucial role in the development of CD8 cytotoxic T cells and anti-tumor immunity. (PMID: 9826581; 23696891)

Protocols

Product Specific Protocols
IF protocol for TNFRSF9/CD137 antibody 98112-1-RRDownload protocol
FC protocol for TNFRSF9/CD137 antibody 98112-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols