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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibody

TNFRSF9/CD137 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241344F3

Cat no : 98112-1-RR

Synonyms

TNFRSF9, ILA, CD137, 4-1BB ligand receptor, 4 1BB ligand receptor

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibo (98112-1-RR)

    FC experiment of human PBMCs using 98112-1-RR

    1x10^6 unstimulated or Con-A-stimulated human PBMCs were surface stained with 0.25 ug Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibody (98112-1-RR, Clone: 241344F3) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug APC Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (APC-65559, Clone: UCHT4). Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibo (98112-1-RR)

    FC experiment of human PBMCs using 98112-1-RR

    1x10^6 unstimulated (black) or Con-A-stimulated human PBMCs (red) were surface stained with 0.25 ug Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibody (98112-1-RR, Clone: 241344F3) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), or Isotype Control (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98112-1-RR

    Affinity and Kinetic Characterization of 98112-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98112-1-RR against Human TNFRSF9/CD137 were performed. The affinity constant is 10.3 nM.

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Tested Applications

Positive FC detected inCon-A-stimulated human PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98112-1-RR targets TNFRSF9/CD137 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 9
Calculated molecular weight 28 kDa
GenBank accession numberBC006196
Gene symbol TNFRSF9/CD137
Gene ID (NCBI) 3604
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD137, also known as TNFRSF9 or 4-1BB, is an inducible T cell surface receptor that belongs to the tumor necrosis factor receptor superfamily. CD137 is a transmembrane protein expressed on the surface of activated T-cells. In addition, activation-dependent expression of CD137 has also been found in B lymphocytes, monocytes, and diverse nonlymphoid cell types. CD137 provides a co-stimulatory signal that enhances the survival, and differentiation of cells, and has a crucial role in the development of CD8 cytotoxic T cells and anti-tumor immunity. (PMID: 9826581; 23696891)

Protocols

Product Specific Protocols
FC protocol for TNFRSF9/CD137 antibody 98112-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols