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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNFSF8 Polyclonal antibody

TNFSF8 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 17852-1-AP

Synonyms

CD153, CD30 L, CD30 ligand, CD30L, CD30LG, TNFSF8

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using TNFSF8 Polyclonal antibody (17852-1-AP)

    WB analysis of MCF-7 using 17852-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 17852-1-AP (TNFSF8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TNFSF8 Polyclonal antibody (17852-1-AP)

    IHC staining of human liver cancer using 17852-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17852-1-AP (TNFSF8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TNFSF8 Polyclonal antibody (17852-1-AP)

    IHC staining of human liver cancer using 17852-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17852-1-AP (TNFSF8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17852-1-AP is TNFSF8 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNFSF8 fusion protein Ag12303 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (ligand) superfamily, member 8
Calculated molecular weight 234 aa, 26 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC093630
Gene symbol TNFSF8
Gene ID (NCBI) 944
RRIDAB_2878452
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TNFSF8, also named as CD30LG and CD153, is a member of the tumor necrosis factor (TNF) receptor superfamily, is a surface antigen used as a clinical marker for Hodgkin lymphoma and related hematologic malignancies. It induces proliferation of T-cells. CD30L enhanced the proliferation of CD3-activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines. For Glycosylation, the MW in WB detection is about 35-40 kDa.

Protocols

Product Specific Protocols
WB protocol for TNFSF8 antibody 17852-1-APDownload protocol
IHC protocol for TNFSF8 antibody 17852-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols