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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNIK Polyclonal antibody

TNIK Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30906-1-AP

Synonyms

TRAF2 and NCK-interacting protein kinase, KIAA0551, EC:2.7.11.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNIK Polyclonal antibody (30906-1-AP)

    WB analysis using 30906-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30906-1-AP (TNIK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TNIK Polyclonal antibody (30906-1-AP)

    WB analysis using 30906-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30906-1-AP (TNIK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using TNIK Polyclonal antibody (30906-1-AP)

    IF Staining of K-562 using 30906-1-AP

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using TNIK antibody (30906-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inCOLO 205 cells, HT-29 cells, K-562 cells
Positive IF/ICC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30906-1-AP targets TNIK in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNIK fusion protein Ag34132 相同性解析による交差性が予測される生物種
Full Name TRAF2 and NCK interacting kinase
Observed molecular weight170 kDa
GenBank accession numberBC150256
Gene symbol TNIK
Gene ID (NCBI) 23043
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Traf2 and NcK interacting kinase (TNiK), a protein with both scaffolding and kinase domains, had been implicated in postsynaptic signaling as well as in regulation of cell proliferation. TNiK is expressed in the nervous system and plays key roles at the synapse and nucleus, In non-neuronal cells, TNiK has been described as a critical component of transcriptional regulatory mechanisms, mediated by Wnt signaling.

Protocols

Product Specific Protocols
WB protocol for TNIK antibody 30906-1-APDownload protocol
IF protocol for TNIK antibody 30906-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols