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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNIK Recombinant antibody

TNIK Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241518D9

Cat no : 84231-4-RR

Synonyms

TRAF2 and NCK-interacting protein kinase, KIAA0551, EC:2.7.11.1, 241518D9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNIK Recombinant antibody (84231-4-RR)

    WB analysis using 84231-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84231-4-RR (TNIK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using TNIK Recombinant antibody (84231-4-RR)

    IF Staining of K-562 using 84231-4-RR

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using TNIK antibody (84231-4-RR, Clone: 241518D9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84231-4-RR

    Affinity and Kinetic Characterization of 84231-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84231-4-RR against Human TNIK were performed. The affinity constant is 2.01 nM.

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Tested Applications

Positive WB detected inT-47D cells, HeLa cells, HEK-293 cells, K-562 cells, COLO 205 cells, Caco-2 cells
Positive IF/ICC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84231-4-RR is TNIK Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TNIK fusion protein Ag34132 相同性解析による交差性が予測される生物種
Full Name TRAF2 and NCK interacting kinase
Observed molecular weight170 kDa
GenBank accession numberBC150256
Gene symbol TNIK
Gene ID (NCBI) 23043
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Traf2- and Nck-interacting kinase (TNIK) is one of the germinal center kinase family members involved in cytoskeleton organization and neuronal dendrite extension (PMID: 24566388). TNIK is an essential regulatory component of the T-cell factor-4 and β-catenin transcriptional complex and is required for the tumor-initiating function of colorectal cancer stem cells (PMID: 10521462).

Protocols

Product Specific Protocols
WB protocol for TNIK antibody 84231-4-RRDownload protocol
IF protocol for TNIK antibody 84231-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols