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KD/KO Validated

TNKS Polyclonal antibody

Name: TNKS Polyclonal antibody
Brand: Proteintech
SKU: 18030-1-AP

TNKS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 18030-1-AP

Synonyms

PARP5A, PARP 5a, EC:2.4.2.30, EC:2.4.2.-, ARTD5

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HeLa using 18030-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18030-1-AP (TNKS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 18030-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 18030-1-AP (TNKS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 18030-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 18030-1-AP (TNKS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 18030-1-AP
IP result of anti-TNKS (IP:18030-1-AP, 4ug; Detection:18030-1-AP 1:500) with Jurkat cells lysate 3200ug.
IHC staining of mouse testis using 18030-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18030-1-AP (TNKS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 18030-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18030-1-AP (TNKS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 18030-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18030-1-AP (TNKS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 18030-1-AP
Immunofluorescent analysis of HepG2 cells, using TNKS antibody 18030-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Cat No. 18030-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, Jurkat cells, MCF-7 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	mouse testis tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IHC	See 2 publications below
IF	See 2 publications below

Product Information

18030-1-AP targets TNKS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TNKS fusion protein Ag12597 相同性解析による交差性が予測される生物種
Full Name	tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
Calculated molecular weight	1327 aa, 142 kDa
Observed molecular weight	150 kDa
GenBank accession number	BC098394
Gene symbol	TNKS
Gene ID (NCBI)	8658
RRID	AB_2303564
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TNKS antibody 18030-1-AP	Download protocol
IHC protocol for TNKS antibody 18030-1-AP	Download protocol
IF protocol for TNKS antibody 18030-1-AP	Download protocol
IP protocol for TNKS antibody 18030-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Mol Cell PARP5A and RNF146 phase separation restrains RIPK1-dependent necroptosis Authors - Shouqiao Hou View Article
		J Biol Chem The dePARylase NUDT16 promotes radiation resistance of cancer cells by blocking SETD3 for degradation via reversing its ADP-ribosylation Authors - Weijun Wu View Article
human	IHC, WB, IF	Oncol Lett Dihydroartemisinin suppresses proliferation, migration, the Wnt/β-catenin pathway and EMT via TNKS in gastric cancer. Authors - Yanmei Ma View Article
human	WB,IHC	Cell Death Dis circ5615 functions as a ceRNA to promote colorectal cancer progression by upregulating TNKS. Authors - Zhifei Ma KD Validated View Article
human	WB	Adv Sci (Weinh) Transferrin Receptor-Mediated Iron Uptake Promotes Colon Tumorigenesis Authors - Hyeoncheol Kim View Article
human	WB	Chem Biol Interact Identification and evaluation of a novel PARP1 inhibitor for the treatment of triple-negative breast cancer Authors - Rong Gong View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNKS Polyclonal antibody

TNKS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 18030-1-AP

WB analysis of Jurkat using 18030-1-AP

WB analysis of MCF-7 using 18030-1-AP

IP experiment of Jurkat using 18030-1-AP

IHC staining of mouse testis using 18030-1-AP

IHC staining of human colon cancer using 18030-1-AP

IHC staining of mouse testis using 18030-1-AP

IF Staining of HepG2 using 18030-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

PARP5A and RNF146 phase separation restrains RIPK1-dependent necroptosis

The dePARylase NUDT16 promotes radiation resistance of cancer cells by blocking SETD3 for degradation via reversing its ADP-ribosylation

Dihydroartemisinin suppresses proliferation, migration, the Wnt/β-catenin pathway and EMT via TNKS in gastric cancer.

circ5615 functions as a ceRNA to promote colorectal cancer progression by upregulating TNKS.

Transferrin Receptor-Mediated Iron Uptake Promotes Colon Tumorigenesis

Identification and evaluation of a novel PARP1 inhibitor for the treatment of triple-negative breast cancer