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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNNI1 Polyclonal antibody

TNNI1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22253-1-AP

Synonyms

SSTNI, TNN1, TNNI1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNNI1 Polyclonal antibody (22253-1-AP)

    WB analysis using 22253-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22253-1-AP (TNNI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using TNNI1 Polyclonal antibody (22253-1-AP)

    IHC staining of human skeletal muscle using 22253-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22253-1-AP (TNNI1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using TNNI1 Polyclonal antibody (22253-1-AP)

    IHC staining of human skeletal muscle using 22253-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22253-1-AP (TNNI1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using TNNI1 Polyclonal antibody (22253-1-AP)

    IHC staining of human heart using 22253-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 22253-1-AP (TNNI1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using TNNI1 Polyclonal antibody (22253-1-AP)

    IHC staining of human heart using 22253-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 22253-1-AP (TNNI1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22253-1-AP is TNNI1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNNI1 fusion protein Ag17749 相同性解析による交差性が予測される生物種
Full Name troponin I type 1 (skeletal, slow)
Calculated molecular weight 187 aa, 22 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC012600
Gene symbol TNNI1
Gene ID (NCBI) 7135
RRIDAB_11182276
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TNNI1 antibody 22253-1-APDownload protocol
IHC protocol for TNNI1 antibody 22253-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols