Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle |
| Positive IHC detected in | human skeletal muscle tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22253-1-AP targets TNNI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TNNI1 fusion protein Ag17749 相同性解析による交差性が予測される生物種 |
| Full Name | troponin I type 1 (skeletal, slow) |
| Calculated molecular weight | 187 aa, 22 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC012600 |
| Gene Symbol | TNNI1 |
| Gene ID (NCBI) | 7135 |
| RRID | AB_11182276 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P19237 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TNNI1 antibody 22253-1-AP | Download protocol |
| IHC protocol for TNNI1 antibody 22253-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |