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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNNI2 Polyclonal antibody

TNNI2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11634-1-AP

Synonyms

AMCD2B, DA2B, FSSV, fsTnI, TNNI2, Troponin I fast skeletal muscle

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNNI2 Polyclonal antibody (11634-1-AP)

    WB analysis using 11634-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using TNNI2 Polyclonal antibody (11634-1-AP)

    WB analysis of mouse skeletal muscle using 11634-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat skeletal muscle tissue using TNNI2 Polyclonal antibody (11634-1-AP)

    WB analysis of rat skeletal muscle using 11634-1-AP

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using TNNI2 Polyclonal antibody (11634-1-AP)

    WB analysis of human kidney using 11634-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using TNNI2 Polyclonal antibody (11634-1-AP)

    WB analysis of human spleen using 11634-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using TNNI2 Polyclonal antibody (11634-1-AP)

    WB analysis of human skeletal muscle using 11634-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using TNNI2 Polyclonal antibody (11634-1-AP)

    IHC staining of mouse skeletal muscle using 11634-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11634-1-AP (TNNI2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using TNNI2 Polyclonal antibody (11634-1-AP)

    IHC staining of human skeletal muscle using 11634-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 11634-1-AP (TNNI2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using TNNI2 Polyclonal antibody (11634-1-AP)

    IHC staining of human skeletal muscle using 11634-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 11634-1-AP (TNNI2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using TNNI2 Polyclonal antibody (11634-1-AP)

    IHC staining of mouse skeletal muscle using 11634-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11634-1-AP (TNNI2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue, human kidney tissue, human spleen tissue, human skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11634-1-AP targets TNNI2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNNI2 fusion protein Ag2208 相同性解析による交差性が予測される生物種
Full Name troponin I type 2 (skeletal, fast)
Calculated molecular weight 182 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC032148
Gene symbol TNNI2
Gene ID (NCBI) 7136
RRIDAB_2206109
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TNNI2 antibody 11634-1-APDownload protocol
IHC protocol for TNNI2 antibody 11634-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols