TNNI2 Polyclonal antibody

Name: TNNI2 Polyclonal antibody
Brand: Proteintech
SKU: 22130-1-AP

TNNI2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 22130-1-AP

Synonyms

Troponin I fast skeletal muscle

Validation Data Gallery

Filter:

AllWBIPIHCIF-P

WB analysis of mouse skeletal muscle using 22130-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22130-1-AP (TNNI2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 22130-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22130-1-AP (TNNI2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
WB analysis of rat skeletal muscle using 22130-1-AP
rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22130-1-AP (TNNI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse skeletal muscle using 22130-1-AP
IP result of anti-TNNI2 (IP:22130-1-AP, 4ug; Detection:22130-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.
IHC staining of human skeletal muscle using 22130-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22130-1-AP (TNNI2 Antibody) at dilution of 1:50.
IHC staining of mouse skeletal muscle using 22130-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22130-1-AP (TNNI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse skeletal muscle using 22130-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TNNI2 antibody (22130-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse skeletal muscle using 22130-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TNNI2 antibody (22130-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 22130-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected in	mouse skeletal muscle tissue
Positive IHC detected in	mouse skeletal muscle tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse skeletal muscle tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:100000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below

Product Information

22130-1-AP targets TNNI2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TNNI2 fusion protein Ag17750 相同性解析による交差性が予測される生物種
Full Name	troponin I type 2 (skeletal, fast)
Calculated molecular weight	182 aa, 21 kDa
Observed molecular weight	21-26 kDa
GenBank accession number	BC032148
Gene symbol	TNNI2
Gene ID (NCBI)	7136
RRID	AB_2879002
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TNNI2 antibody 22130-1-AP	Download protocol
IHC protocol for TNNI2 antibody 22130-1-AP	Download protocol
IF protocol for TNNI2 antibody 22130-1-AP	Download protocol
IP protocol for TNNI2 antibody 22130-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Ann Surg Oncol Troponin I2 as a Specific Biomarker for Prediction of Peritoneal Metastasis in Gastric Cancer. Authors - Koichi Sawaki View Article
mouse	WB	Sci Rep A missense mutation in the RSRSP stretch of Rbm20 causes dilated cardiomyopathy and atrial fibrillation in mice. Authors - Kensuke Ihara View Article
mouse	WB	Front Physiol Lipidomic Analyses Reveal Specific Alterations of Phosphatidylcholine in Dystrophic Mdx Muscle. Authors - William J Valentine View Article
mouse	WB	J Muscle Res Cell Motil Differential regulation of Actn2 and Actn3 expression during unfolded protein response in C2C12 myotubes. Authors - Nagakatsu Harada View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNNI2 Polyclonal antibody

TNNI2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Validation Data Gallery

WB analysis of mouse skeletal muscle using 22130-1-AP

WB analysis using 22130-1-AP

WB analysis of rat skeletal muscle using 22130-1-AP

IP experiment of mouse skeletal muscle using 22130-1-AP

IHC staining of human skeletal muscle using 22130-1-AP

IHC staining of mouse skeletal muscle using 22130-1-AP

IF Staining of mouse skeletal muscle using 22130-1-AP

IF Staining of mouse skeletal muscle using 22130-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Troponin I2 as a Specific Biomarker for Prediction of Peritoneal Metastasis in Gastric Cancer.

A missense mutation in the RSRSP stretch of Rbm20 causes dilated cardiomyopathy and atrial fibrillation in mice.

Lipidomic Analyses Reveal Specific Alterations of Phosphatidylcholine in Dystrophic Mdx Muscle.

Differential regulation of Actn2 and Actn3 expression during unfolded protein response in C2C12 myotubes.