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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNNT1 Monoclonal antibody (68631-1-Ig)

    WB analysis using 68631-1-Ig (same clone as 68631-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68631-1-Ig (TNNT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68631-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using TNNT1 Monoclonal antibody (68631-1-Ig)

    IHC staining of mouse skeletal muscle using 68631-1-Ig (same clone as 68631-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68631-1-Ig (TNNT1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68631-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using TNNT1 Monoclonal antibody (68631-1-Ig)

    IHC staining of rat skeletal muscle using 68631-1-Ig (same clone as 68631-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68631-1-Ig (TNNT1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68631-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using TNNT1 Monoclonal antibody (68631-1-Ig)

    IHC staining of rat skeletal muscle using 68631-1-Ig (same clone as 68631-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68631-1-Ig (TNNT1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68631-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using TNNT1 Monoclonal antibody (68631-1-Ig)

    IF Staining of hTERT-RPE1 using 68631-1-Ig (same clone as 68631-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using TNNT1 antibody (68631-1-Ig, Clone: 1B1D5 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68631-1-PBS in a different storage buffer formulation.

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TNNT1 Monoclonal antibody, PBS Only

TNNT1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68631-1-PBS
Clone No.1B1D5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

TNTS, TNT, STNT, ANM

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68631-1-PBS targets TNNT1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TNNT1 fusion protein Ag8919 相同性解析による交差性が予測される生物種
Full Name troponin T type 1 (skeletal, slow)
Calculated molecular weight252aa,27 kDa; 192aa,21 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC022086
Gene Symbol TNNT1
Gene ID (NCBI) 7138
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13805
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.