Validation Data Gallery

Filter: AllWBIHCIF-PIF-Fro

WB analysis of mouse heart using 15513-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 15513-1-AP (Cardiac Troponin T antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 15513-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 15513-1-AP (TNNT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 15513-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 15513-1-AP (Cardiac Troponin T antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 15513-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15513-1-AP (Cardiac Troponin T antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of mouse heart using 15513-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15513-1-AP (Cardiac Troponin T antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 15513-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15513-1-AP (Cardiac Troponin T antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 15513-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15513-1-AP (Cardiac Troponin T antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 15513-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15513-1-AP (Cardiac Troponin T antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse heart using 15513-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Cardiac Troponin T antibody (15513-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse heart using 15513-1-AP
Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (red, Cat. No 15513-1-AP), CD31/PECAM-1 (magenta), and Arl13B (green, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (blue). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody. CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody. The Image and figure legends are intellectual property of @LAF_in_the_LAB.
×
Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (red, Cat. No 15513-1-AP), CD31/PECAM-1 (magenta), and Arl13B (green, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (blue). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody. CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody. The Image and figure legends are intellectual property of @LAF_in_the_LAB.
IF Staining of mouse heart using 15513-1-AP
Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (magenta, Cat. No 15513-1-AP), CD31/PECAM-1 (white), and Arl13B (yellow, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (cyan). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to magenta.CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody and psedocolored to yellow. The Image and figure legends are intellectual property of @LAF_in_the_LAB.
×
Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (magenta, Cat. No 15513-1-AP), CD31/PECAM-1 (white), and Arl13B (yellow, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (cyan). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to magenta.CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody and psedocolored to yellow. The Image and figure legends are intellectual property of @LAF_in_the_LAB.

Cardiac Troponin T Polyclonal antibody

Cardiac Troponin T Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Cat No. 15513-1-AP

Publications(65)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Synonyms

TNNT2, cTnT, Troponin T, cardiac muscle

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (65)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IHC detected in	mouse heart tissue, mouse skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse heart tissue
Positive IF-Fro detected in	mouse heart tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)-FRO	IF-FRO : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 16 publications below
IHC	See 3 publications below
IF	See 46 publications below
FC	See 3 publications below

Product Information

15513-1-AP targets Cardiac Troponin T in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cardiac Troponin T fusion protein Ag7716 相同性解析による交差性が予測される生物種
Full Name	troponin T type 2 (cardiac)
Calculated molecular weight	36 kDa
Observed molecular weight	34-40 kDa
GenBank accession number	BC002653
Gene Symbol	Cardiac Troponin T
Gene ID (NCBI)	7139
RRID	AB_2206563
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P45379
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Cardiac Troponin T antibody 15513-1-AP	Download protocol
IHC protocol for Cardiac Troponin T antibody 15513-1-AP	Download protocol
IF protocol for Cardiac Troponin T antibody 15513-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Nature Brahma safeguards canalization of cardiac mesoderm differentiation. Authors - Swetansu K Hota View Article
pig	IF	Cell Stem Cell Generation of a humanized mesonephros in pigs from induced pluripotent stem cells via embryo complementation Authors - Jiaowei Wang View Article
human	IF	Sci Transl Med Precise genomic editing of pathogenic mutations in RBM20 rescues dilated cardiomyopathy Authors - Takahiko Nishiyama View Article
human	IF	ACS Nano Intrinsic Color Sensing System Allows for Real-Time Observable Functional Changes on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Authors - Yiqi Gong View Article
mouse	IF	Acta Pharm Sin B Hippo pathway-manipulating neutrophil-mimic hybrid nanoparticles for cardiac ischemic injury via modulation of local immunity and cardiac regeneration Authors - Qiaozi Wang View Article
mouse	IHC	Dev Cell A Multifunctional Protein, EWS, Is Essential for Early Brown Fat Lineage Determination. Authors - Park Jun Hong JH View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse heart using 15513-1-AP

WB analysis of human heart using 15513-1-AP

WB analysis of rat heart using 15513-1-AP

WB analysis of mouse skeletal muscle using 15513-1-AP

IHC staining of mouse heart using 15513-1-AP

IHC staining of mouse heart using 15513-1-AP

IHC staining of mouse skeletal muscle using 15513-1-AP

IHC staining of mouse skeletal muscle using 15513-1-AP

IF Staining of mouse heart using 15513-1-AP

IF Staining of mouse heart using 15513-1-AP

IF Staining of mouse heart using 15513-1-AP

Cardiac Troponin T Polyclonal antibody

Cardiac Troponin T Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Brahma safeguards canalization of cardiac mesoderm differentiation.

Generation of a humanized mesonephros in pigs from induced pluripotent stem cells via embryo complementation

Precise genomic editing of pathogenic mutations in RBM20 rescues dilated cardiomyopathy

Intrinsic Color Sensing System Allows for Real-Time Observable Functional Changes on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Hippo pathway-manipulating neutrophil-mimic hybrid nanoparticles for cardiac ischemic injury via modulation of local immunity and cardiac regeneration

A Multifunctional Protein, EWS, Is Essential for Early Brown Fat Lineage Determination.