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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNNT3 Polyclonal antibody

TNNT3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19729-1-AP

Synonyms

Troponin T-FS, Troponin T3 skeletal fast, Fast skeletal muscle troponin T, Beta-TnTF

Validation Data Gallery

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  • Western Blot (WB) analysis of rat skeletal muscle tissue using TNNT3 Polyclonal antibody (19729-1-AP)

    WB analysis of rat skeletal muscle using 19729-1-AP

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19729-1-AP (TNNT3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using TNNT3 Polyclonal antibody (19729-1-AP)

    WB analysis of mouse skeletal muscle using 19729-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19729-1-AP (TNNT3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using TNNT3 Polyclonal antibody (19729-1-AP)

    IHC staining of mouse skeletal muscle using 19729-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19729-1-AP (TNNT3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using TNNT3 Polyclonal antibody (19729-1-AP)

    IHC staining of mouse skeletal muscle using 19729-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19729-1-AP (TNNT3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using TNNT3 Polyclonal antibody (19729-1-AP)

    IHC staining of human skeletal muscle using 19729-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 19729-1-AP (TNNT3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using TNNT3 Polyclonal antibody (19729-1-AP)

    IHC staining of human skeletal muscle using 19729-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 19729-1-AP (TNNT3 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inrat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19729-1-AP targets TNNT3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name troponin T type 3 (skeletal, fast)
Calculated molecular weight 32 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberNM_001042780
Gene symbol TNNT3
Gene ID (NCBI) 7140
RRIDAB_2878601
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TNNT3 antibody 19729-1-APDownload protocol
IHC protocol for TNNT3 antibody 19729-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols