TNPO1 Polyclonal antibody

TNPO1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20679-1-AP

Synonyms

transportin 1, Transportin1, Transportin-1, KPN B2, Karyopherin beta-2


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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, L02 cells, mouse liver tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20679-1-AP targets TNPO1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name transportin 1
Calculated molecular weight 102 kDa
Observed molecular weight 102 kDa
GenBank accession numberNM_002270
Gene symbol Transportin-1
Gene ID (NCBI) 3842
RRIDAB_10694291
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TNPO1, also named as KPNB2, MIP1, TRN and MIP, belongs to the importin beta family. TNPO1 functions in nuclear protein import as nuclear transport receptor. It serves as receptor for nuclear localization signals (NLS) in cargo substrates. TNPO1 is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. TNPO1 is involved in nuclear import of M9-containing proteins. In vitro, it binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. It is involved in hnRNP A1/A2 nuclear export. TNPO1 mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. It binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, it mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. The antibody is specific to TNPO1.

Protocols

Product Specific Protocols
WB protocol for TNPO1 antibody 20679-1-APDownload protocol
IHC protocol for TNPO1 antibody 20679-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

PLoS Genet

MxB inhibits long interspersed element type 1 retrotransposition.

Authors - Yu Huang
humanIF

Sci Rep

Nuclear RNA transcript levels modulate nucleocytoplasmic distribution of ALS/FTD-associated protein FUS.

Authors - Yueh-Lin Tsai
humanIF

Cell Rep

Antiviral Immune Response as a Trigger of FUS Proteinopathy in Amyotrophic Lateral Sclerosis.

Authors - Tatyana A Shelkovnikova
humanWB

Small

Effects of Puerarin-Loaded Tetrahedral Framework Nucleic Acids on Osteonecrosis of the Femoral Head

Authors - Yuxuan Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

María (Verified Customer) (02-08-2022)

Works good for WB (1:1000). For IF, some background but works ok (1:100)

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: RPE-1/MSC