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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNRC18 Polyclonal antibody (32165-1-AP)

    WB analysis using 32165-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32165-1-AP (TNRC18 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TNRC18 Polyclonal antibody (32165-1-AP)

    WB analysis using 32165-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32165-1-AP (TNRC18 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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TNRC18 Polyclonal antibody

TNRC18 Polyclonal Antibody for WB, ELISA
Cat No. 32165-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Trinucleotide repeat-containing gene 18 protein, Trinucleotide Repeat Containing 18, Long CAG trinucleotide repeat-containing gene 79 protein, KIAA1856, CAGL79

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBxPC-3 cells, HepG2 cells, MDA-MB-453 cells, mouse kidney tissue, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32165-1-AP targets TNRC18 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNRC18 fusion protein Ag37483 相同性解析による交差性が予測される生物種
Full Name trinucleotide repeat containing 18
Calculated molecular weight31 kDa
Observed molecular weight29 kDa
GenBank accession numberBC131808
Gene Symbol TNRC18
Gene ID (NCBI) 84629
RRIDAB_3670212
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO15417
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Trinucleotide repeat containing 18 (TNRC18, also known as CAGL79 and KIAA1856) is a nucleic-acid-binding protein, which recognizes H3K9me3 to mediate the silencing of ERV class I (ERV1) elements and acts as a chromatin-associated regulator of ERVs (PMID: 27980689; 37938770). It is located in the cytosol, mitochondrion, and nucleus (PMID: 19262598). The mutation in TNRC18 may cause Fazio-Londe disease (PMID: 38188848).

Protocols

Product Specific Protocols
WB protocol for TNRC18 antibody 32165-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols