• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNRC4 Polyclonal antibody

TNRC4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25353-1-AP

Synonyms

Bruno like protein 1, BRUNOL1, CAG repeat protein 4, CAGH4, CELF 3, CELF3, ERDA4, ETR 1, RNA binding protein BRUNOL 1, TNRC4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse lung tissue using TNRC4 Polyclonal antibody (25353-1-AP)

    WB analysis of mouse lung using 25353-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 25353-1-AP (TNRC4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using TNRC4 Polyclonal antibody (25353-1-AP)

    WB analysis of mouse spleen using 25353-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25353-1-AP (TNRC4 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human testis tissue using TNRC4 Polyclonal antibody (25353-1-AP)

    IHC staining of human testis using 25353-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25353-1-AP (TNRC4 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using TNRC4 Polyclonal antibody (25353-1-AP)

    IHC staining of human cerebellum using 25353-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25353-1-AP (TNRC4 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TNRC4 Polyclonal antibody (25353-1-AP)

    IHC staining of human testis using 25353-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25353-1-AP (TNRC4 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using TNRC4 Polyclonal antibody (25353-1-AP)

    IHC staining of human cerebellum using 25353-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25353-1-AP (TNRC4 Antibody) at dilution of 1:50 (under 10x lens).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inmouse lung tissue, mouse spleen tissue
Positive IHC detected inhuman testis tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25353-1-AP is TNRC4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNRC4 fusion protein Ag18601 相同性解析による交差性が予測される生物種
Full Name trinucleotide repeat containing 4
Calculated molecular weight 464 aa, 51 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC052491
Gene symbol TNRC4
Gene ID (NCBI) 11189
RRIDAB_2880041
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TNRC4, also named as BRUNOL1, CAGH4, ERDA4, or TNRC4, is a 465 amino acid protein, which contains three RRM (RNA recognition motif) domains and belongs to the CELF/BRUNOL family. TNRC4 localizes in the nucleus and is expressed in brain. TNRC4 as a RNA-binding protein is involved in the regulation of pre-mRNA alternative splicing and mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing.

Protocols

Product Specific Protocols
WB protocol for TNRC4 antibody 25353-1-APDownload protocol
IHC protocol for TNRC4 antibody 25353-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols