Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse heart tissue |
| Positive IHC detected in | mouse heart tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
20054-1-AP targets TNS1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | tensin 1 |
| Calculated molecular weight | 186 kDa |
| Observed molecular weight | 186 kDa |
| GenBank accession number | NM_022648 |
| Gene Symbol | TNS1 |
| Gene ID (NCBI) | 7145 |
| RRID | AB_2878636 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HBL0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TNS1, also named as TNS, may be involved in cell migration, cartilage development and in linking signal transduction pathways to the cytoskeleton. The antibody recognizes near the N-term of TNS1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TNS1 antibody 20054-1-AP | Download protocol |
| IHC protocol for TNS1 antibody 20054-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) LncRNA MAGI2-AS3 inhibits bladder cancer progression by targeting the miR-31-5p/TNS1 axis. | ||
EBioMedicine Transcriptomics based multi-dimensional characterization and drug screen in esophageal squamous cell carcinoma. | ||
Reproduction Deep RNA sequencing analysis of syncytialization-related genes during BeWo cell fusion. | ||
Exp Cell Res MicroRNA-522-3p promotes brain metastasis in non-small cell lung cancer by targeting Tensin 1 and modulating blood-brain barrier permeability |