- Featured Product
- KD/KO Validated
TNS4 Polyclonal antibody
TNS4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, CoIP, ELISA, Cell treatment
Conjugate
Unconjugated
Cat no : 11580-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, human placenta tissue, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A549 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 3 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
CoIP | See 1 publications below |
Product Information
11580-1-AP targets TNS4 in WB, IHC, IF/ICC, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TNS4 fusion protein Ag2169 相同性解析による交差性が予測される生物種 |
Full Name | tensin 4 |
Calculated molecular weight | 715 aa, 77 kDa |
Observed molecular weight | 77 kDa |
GenBank accession number | BC018706 |
Gene symbol | TNS4 |
Gene ID (NCBI) | 84951 |
RRID | AB_10640582 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TNS4 (Tensin-4), also known as COOH-terminus tensin-like protein, is a distinctive member with the smallest molecular mass and lacks the N-terminal common region found in other family members. TNS4 plays a crucial role in CRC tumorigenesis, and that suppression of TNS4 would be an effective therapeutic strategy in treating a subset of cetuximab-refractory CRC patients including KRAS activating mutations (PMID: 31409052). TNS4 has been implicated in numerous vital biological processes, including proliferation, migration, cell adhesion, and apoptosis (PMID: 38164166).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for TNS4 antibody 11580-1-AP | Download protocol |
IHC protocol for TNS4 antibody 11580-1-AP | Download protocol |
IF protocol for TNS4 antibody 11580-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Bioeng Biotechnol Smarce1 and Tensin 4 Are Putative Modulators of Corneoscleral Stiffness. | ||
Pathol Res Pract Tensin4 promotes invasion and migration of gastric cancer cells via regulating AKT/GSK-3β/snail signaling pathway.
| ||
Antioxid Redox Signal A novel lncRNA FUAT1/TNS4 axis confers chemoresistance by suppressing ROS-mediated apoptosis in gastric cancer | ||
Int J Biol Sci Hypoxia-driven TNS4 fosters HNSCC tumorigenesis by stabilizing integrin α5β1 complex and triggering FAK-mediated Akt and TGFβ signaling pathways |