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  • Featured Product
  • KD/KO Validated

TOAG1 Polyclonal antibody

TOAG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21564-1-AP

Synonyms

C3orf23, TCAIM, T-cell activation inhibitor, TOAG1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using TOAG1 Polyclonal antibody (21564-1-AP)

    WB analysis of HEK-293 using 21564-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21564-1-AP (TOAG1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using TOAG1 Polyclonal antibody (21564-1-AP)

    IHC staining of human liver using 21564-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21564-1-AP (TOAG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using TOAG1 Polyclonal antibody (21564-1-AP)

    IHC staining of human liver using 21564-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21564-1-AP (TOAG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21564-1-AP targets TOAG1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOAG1 fusion protein Ag16067 相同性解析による交差性が予測される生物種
Full Name chromosome 3 open reading frame 23
Calculated molecular weight 496 aa, 58 kDa
Observed molecular weight 35-41 kDa
GenBank accession numberBC101060
Gene symbol TOAG1
Gene ID (NCBI) 285343
RRIDAB_2878885
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOAG1, also named as TCAIM and C3orf23, is a mitochondrial protein which regulates T-cell apoptosis. It is a specific and reproducible marker for quiescence. TOAG1 has two isoforms with MW 41 kDa and 58 kDa.

Protocols

Product Specific Protocols
WB protocol for TOAG1 antibody 21564-1-APDownload protocol
IHC protocol for TOAG1 antibody 21564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

FEBS J

A novel role of TCAIM: suppressing renal carcinoma growth and enhancing its sensitivity to sunitinib

Authors - Zhuo Ye
  • KD Validated
View Article