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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TOE1 Polyclonal antibody

TOE1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30221-1-AP

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TOE1 Polyclonal antibody (30221-1-AP)

    WB analysis using 30221-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30221-1-AP (TOE1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TOE1 Polyclonal antibody (30221-1-AP)

    IHC staining of human stomach cancer using 30221-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30221-1-AP (TOE1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TOE1 Polyclonal antibody (30221-1-AP)

    IF Staining of HeLa using 30221-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOE1 antibody (30221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30221-1-AP targets TOE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOE1 fusion protein Ag31470 相同性解析による交差性が予測される生物種
Full Name target of EGR1, member 1 (nuclear)
Calculated molecular weight 510 aa, 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC009364
Gene symbol TOE1
Gene ID (NCBI) 114034
RRIDAB_2935533
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOE1, also known as hCaf1z, belongs to the DEDD family of deadenylases. TOE1 was initially identified as a direct target gene of ERG1, an immediate early transcription factor. TOE1 encodes an Asp-Glu-Asp-Asp deadenylase that specifically localizes to Cajal bodies in the nucleus, where assembly and maturation of small nuclear ribonucleoprotein particles, and telomerase ribonucleoprotein complex take place (PMID: 36257135). TOE1 has 2 isoforms with the molecular mass of 48 and 57 kDa.

Protocols

Product Specific Protocols
WB protocol for TOE1 antibody 30221-1-APDownload protocol
IHC protocol for TOE1 antibody 30221-1-APDownload protocol
IF protocol for TOE1 antibody 30221-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols