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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TOLLIP Recombinant antibody, PBS Only

TOLLIP Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242098E3

Cat no : 84711-5-PBS

Synonyms

Toll-interacting protein, toll interacting protein, IL 1RAcPIP, 242098E3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TOLLIP Recombinant antibody (84711-5-RR)

    WB analysis using 84711-5-RR (same clone as 84711-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84711-5-RR (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84711-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TOLLIP Recombinant antibody (84711-5-RR)

    IF Staining of HepG2 using 84711-5-RR (same clone as 84711-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TOLLIP antibody (84711-5-RR, Clone: 242098E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84711-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using TOLLIP Recombinant antibody (84711-5-RR)

    FC experiment of U2OS using 84711-5-RR (same clone as 84711-5-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug TOLLIP Recombinant antibody (84711-5-RR, Clone:242098E3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84711-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84711-5-RR

    Affinity and Kinetic Characterization of 84711-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84711-5-RR against Human TOLLIP were performed. The affinity constant is 0.309 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84711-5-PBS targets TOLLIP in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TOLLIP fusion protein Ag1864 相同性解析による交差性が予測される生物種
Full Name toll interacting protein
Calculated molecular weight 30 kDa
Observed molecular weight30 kDa
GenBank accession numberBC012057
Gene symbol TOLLIP
Gene ID (NCBI) 54472
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TOLLIP (Toll interacting protein), is an inhibitory adaptor protein. Tollip is a negative regulator of IL-1R and Toll-like receptors signalling pathway, involved in inflammatory immune response and inhibiting IRAK1 phosphorylation and kinase activity (PMID:11441107, PMID:10854325, PMID:11751856). The TOLLIP-dependent selective autophagy pathway plays a role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851).