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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TOLLIP Recombinant antibody

TOLLIP Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242098C6

Cat no : 84711-6-RR

Synonyms

Toll-interacting protein, toll interacting protein, IL 1RAcPIP, 242098C6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TOLLIP Recombinant antibody (84711-6-RR)

    WB analysis using 84711-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84711-6-RR (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inLNCaP cells, HepG2 cells, PC-3 cells, HCT 116 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84711-6-RR targets TOLLIP in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TOLLIP fusion protein Ag1864 相同性解析による交差性が予測される生物種
Full Name toll interacting protein
Calculated molecular weight 30 kDa
Observed molecular weight30 kDa
GenBank accession numberBC012057
Gene symbol TOLLIP
Gene ID (NCBI) 54472
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOLLIP (Toll interacting protein), is an inhibitory adaptor protein. Tollip is a negative regulator of IL-1R and Toll-like receptors signalling pathway, involved in inflammatory immune response and inhibiting IRAK1 phosphorylation and kinase activity (PMID:11441107, PMID:10854325, PMID:11751856). The TOLLIP-dependent selective autophagy pathway plays a role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851).

Protocols

Product Specific Protocols
WB protocol for TOLLIP antibody 84711-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols