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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TOM20 Monoclonal antibody (66777-1-Ig)

    WB analysis using 66777-1-Ig (same clone as 66777-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66777-1-Ig (TOM20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66777-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TOM20 Monoclonal antibody (66777-1-Ig)

    IHC staining of human liver cancer using 66777-1-Ig (same clone as 66777-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66777-1-Ig (TOM20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66777-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TOM20 Monoclonal antibody (66777-1-Ig)

    IHC staining of human breast cancer using 66777-1-Ig (same clone as 66777-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66777-1-Ig (TOM20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66777-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TOM20 Monoclonal antibody (66777-1-Ig)

    IHC staining of human breast cancer using 66777-1-Ig (same clone as 66777-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66777-1-Ig (TOM20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66777-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TOM20 Monoclonal antibody (66777-1-Ig)

    IHC staining of human liver cancer using 66777-1-Ig (same clone as 66777-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66777-1-Ig (TOM20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66777-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TOM20 Monoclonal antibody (66777-1-Ig)

    IF Staining of HepG2 using 66777-1-Ig (same clone as 66777-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOM20 antibody (66777-1-Ig, Clone: 1D6F5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66777-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TOM20 Monoclonal antibody (66777-1-Ig)

    IF Staining of HepG2 using 66777-1-Ig (same clone as 66777-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOM20 antibody (66777-1-Ig, Clone: 1D6F5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 66777-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using TOM20 Monoclonal antibody (66777-1-Ig)

    FC experiment of HeLa using 66777-1-Ig (same clone as 66777-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TOM20 (66777-1-Ig, Clone:1D6F5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66777-1-PBS in a different storage buffer formulation.

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TOM20 Monoclonal antibody, PBS Only

TOM20 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66777-1-PBS
Clone No.1D6F5

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

TOMM20, TOMM 20, tom 20, MOM19, MAS20

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66777-1-PBS targets TOM20 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TOM20 fusion protein Ag2378 相同性解析による交差性が予測される生物種
Full Name translocase of outer mitochondrial membrane 20 homolog (yeast)
Calculated molecular weight 145 aa, 16 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC000882
Gene Symbol TOM20
Gene ID (NCBI) 9804
RRIDAB_2882123
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15388
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.