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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TOM20 Recombinant antibody

TOM20 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, chicken, zebrafish

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D22

Cat no : 80501-1-RR

Synonyms

TOMM20, TOMM 20, tom 20, MOM19, MAS20

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TOM20 Recombinant antibody (80501-1-RR)

    WB analysis using 80501-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80501-1-RR (TOM20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of zebrafish tissue using TOM20 Recombinant antibody (80501-1-RR)

    WB analysis of zebrafish using 80501-1-RR

    zebrafish tissue were subjected to SDS PAGE followed by western blot with 80501-1-RR (TOM20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using TOM20 Recombinant antibody (80501-1-RR)

    IHC staining of human liver using 80501-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80501-1-RR (TOM20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using TOM20 Recombinant antibody (80501-1-RR)

    IHC staining of human liver using 80501-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80501-1-RR (TOM20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TOM20 Recombinant antibody (80501-1-RR)

    IF Staining of HepG2 using 80501-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOM20 antibody (80501-1-RR, Clone: 1D22 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 80501-1-RR

    Affinity and Kinetic Characterization of 80501-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80501-1-RR against Human TOM20 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, zebrafish tissue, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, pig brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80501-1-RR targets TOM20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, chicken, zebrafish samples.

Tested Reactivity human, mouse, rat, pig, chicken, zebrafish
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TOM20 fusion protein Ag2378 相同性解析による交差性が予測される生物種
Full Name translocase of outer mitochondrial membrane 20 homolog (yeast)
Calculated molecular weight 145 aa, 16 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC000882
Gene symbol TOM20
Gene ID (NCBI) 9804
RRIDAB_2918897
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.

Protocols

Product Specific Protocols
WB protocol for TOM20 antibody 80501-1-RRDownload protocol
IHC protocol for TOM20 antibody 80501-1-RRDownload protocol
IF protocol for TOM20 antibody 80501-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols