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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TOM70 Monoclonal antibody (66593-1-Ig)

    WB analysis using 66593-1-Ig (same clone as 66593-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66593-1-Ig (TOM70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TOM70 Monoclonal antibody (66593-1-Ig)

    IHC staining of mouse brain using 66593-1-Ig (same clone as 66593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TOM70 Monoclonal antibody (66593-1-Ig)

    IHC staining of mouse brain using 66593-1-Ig (same clone as 66593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using TOM70 Monoclonal antibody (66593-1-Ig)

    IHC staining of human brain using 66593-1-Ig (same clone as 66593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using TOM70 Monoclonal antibody (66593-1-Ig)

    IHC staining of human brain using 66593-1-Ig (same clone as 66593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TOM70 Monoclonal antibody (66593-1-Ig)

    IF Staining of HeLa using 66593-1-Ig (same clone as 66593-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66593-1-Ig (TOM70 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

TOM70 Monoclonal antibody, PBS Only

TOM70 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66593-1-PBS
Clone No.1H8A1

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

TOMM70A, Translocase of outer mitochondrial membrane protein 70, TOMM70, TOMM, KIAA0719

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66593-1-PBS targets TOM70 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TOM70 fusion protein Ag6027 相同性解析による交差性が予測される生物種
Full Name translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)
Calculated molecular weight 67 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC052994
Gene Symbol TOM70
Gene ID (NCBI) 9868
RRIDAB_2881953
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO94826
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TOM70 is a mitochondrial import receptor responsible for the import of cytosol synthesized precursor proteins into the mitochondria. Tom70 is a member of the tetratricopeptide repeat (TPR) co-chaperone family and contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90. Recently overexpression of TOM70 has been linked to chemoresistance to drugs in breast cancer cell lines. It is also believed that Tom70 is significantly associated with breast cancer relapse and may predict the recurrence of ovarian cancer.