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Tom22 Polyclonal antibody

Tom22 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11278-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

TOMM22, MSTP065, MST065, hTom22, 1C9 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa, K-562 cells, HeLa cells, mouse liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11278-1-AP targets Tom22 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Tom22 fusion protein Ag1797 相同性解析による交差性が予測される生物種
Full Name translocase of outer mitochondrial membrane 22 homolog (yeast)
Calculated molecular weight 15 kDa
Observed molecular weight 15-22 kDa
GenBank accession numberBC009363
Gene Symbol TOMM22/Tom22
Gene ID (NCBI) 56993
RRIDAB_2207542
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NS69
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tom22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises  the  22 kDa  protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).

Protocols

Product Specific Protocols
WB protocol for Tom22 antibody 11278-1-APDownload protocol
IHC protocol for Tom22 antibody 11278-1-APDownload protocol
IF protocol for Tom22 antibody 11278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

Tom20 senses iron-activated ROS signaling to promote melanoma cell pyroptosis.

Authors - Bo Zhou
humanWB,IF

Cell Discov

Dynamic O-GlcNAcylation coordinates ferritinophagy and mitophagy to activate ferroptosis.

Authors - Fan Yu
humanWB

J Genet Genomics

HPDL deficiency causes a neuromuscular disease by impairing the mitochondrial respiration.

Authors - Yu Sun
humanWB

Mol Biol Cell

Mitochondrial E3 ubiquitin ligase MARCH5 controls mitochondrial fission and cell sensitivity to stress-induced apoptosis through regulation of MiD49 protein.

Authors - Shan Xu
humanWB

J Hum Genet

Mutations in TOMM70 lead to multi-OXPHOS deficiencies and cause severe anemia, lactic acidosis, and developmental delay.

Authors - Xiujuan Wei
humanWB

EMBO Rep

UBXD8 mediates mitochondria-associated degradation to restrain apoptosis and mitophagy

Authors - Jing Zheng
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