Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa, K-562 cells, HeLa cells, mouse liver tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
| IF | See 3 publications below |
Product Information
11278-1-AP targets Tom22 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Tom22 fusion protein Ag1797 相同性解析による交差性が予測される生物種 |
| Full Name | translocase of outer mitochondrial membrane 22 homolog (yeast) |
| Calculated molecular weight | 15 kDa |
| Observed molecular weight | 15-22 kDa |
| GenBank accession number | BC009363 |
| Gene Symbol | TOMM22/Tom22 |
| Gene ID (NCBI) | 56993 |
| RRID | AB_2207542 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NS69 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tom22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises the 22 kDa protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Tom22 antibody 11278-1-AP | Download protocol |
| IHC protocol for Tom22 antibody 11278-1-AP | Download protocol |
| IF protocol for Tom22 antibody 11278-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Discov Dynamic O-GlcNAcylation coordinates ferritinophagy and mitophagy to activate ferroptosis. | ||
J Genet Genomics HPDL deficiency causes a neuromuscular disease by impairing the mitochondrial respiration. | ||
Mol Biol Cell Mitochondrial E3 ubiquitin ligase MARCH5 controls mitochondrial fission and cell sensitivity to stress-induced apoptosis through regulation of MiD49 protein. | ||
J Hum Genet Mutations in TOMM70 lead to multi-OXPHOS deficiencies and cause severe anemia, lactic acidosis, and developmental delay. | ||
EMBO Rep UBXD8 mediates mitochondria-associated degradation to restrain apoptosis and mitophagy |