Validation Data Gallery
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Tested Applications
| Positive WB detected in | A375 cells, HeLa cells, PC-3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 2 publications below |
Product Information
12196-1-AP targets TOMM34 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TOMM34 fusion protein Ag2834 相同性解析による交差性が予測される生物種 |
| Full Name | translocase of outer mitochondrial membrane 34 |
| Calculated molecular weight | 309 aa, 35 kDa |
| Observed molecular weight | 31-35 kDa |
| GenBank accession number | BC014907 |
| Gene Symbol | TOMM34 |
| Gene ID (NCBI) | 10953 |
| RRID | AB_2240906 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15785 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TOMM34 antibody 12196-1-AP | Download protocol |
| IHC protocol for TOMM34 antibody 12196-1-AP | Download protocol |
| IP protocol for TOMM34 antibody 12196-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Neuropathol The proteome of granulovacuolar degeneration and neurofibrillary tangles in Alzheimer's disease. | ||
Free Radic Biol Med Proteomic analysis of the glutathione-deficient LEGSKO mouse lens reveals activation of EMT signaling, loss of lens specific markers, and changes in stress response proteins. | ||
Pathol Oncol Res High Expression of Tomm34 and Its Correlations With Clinicopathology in Oral Squamous Cell Carcinoma. | ||
J Oral Pathol Med TOMM34 promotes cell proliferation, migration and invasion of OSCC and modulates mitochondrial function
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