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KD/KO Validated

TOMM34 Polyclonal antibody

Name: TOMM34 Polyclonal antibody
Brand: Proteintech
SKU: 12196-1-AP

TOMM34 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12196-1-AP

Synonyms

hTom34, HTOM34P, TOM34, TOMM34, URCC3

Validation Data Gallery

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AllWBIPIHC

WB analysis using 12196-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12196-1-AP (TOMM34 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12196-1-AP
WB result of TOMM34 antibody (12196-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOMM34 transfected HeLa cells.
IP experiment of HeLa using 12196-1-AP
IP result of anti-TOMM34 (IP:12196-1-AP, 4ug; Detection:12196-1-AP 1:2000) with HeLa cells lysate 1320 ug.
IHC staining of human urothelial carcinoma using 12196-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12196-1-AP (TOMM34 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 12196-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12196-1-AP (TOMM34 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 12196-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A375 cells, HeLa cells, PC-3 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human urothelial carcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 2 publications below

Product Information

12196-1-AP targets TOMM34 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TOMM34 fusion protein Ag2834 相同性解析による交差性が予測される生物種
Full Name	translocase of outer mitochondrial membrane 34
Calculated molecular weight	309 aa, 35 kDa
Observed molecular weight	31-35 kDa
GenBank accession number	BC014907
Gene symbol	TOMM34
Gene ID (NCBI)	10953
RRID	AB_2240906
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TOMM34 antibody 12196-1-AP	Download protocol
IHC protocol for TOMM34 antibody 12196-1-AP	Download protocol
IP protocol for TOMM34 antibody 12196-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	IHC	Acta Neuropathol The proteome of granulovacuolar degeneration and neurofibrillary tangles in Alzheimer's disease. Authors - David C Hondius View Article
mouse	WB	Free Radic Biol Med Proteomic analysis of the glutathione-deficient LEGSKO mouse lens reveals activation of EMT signaling, loss of lens specific markers, and changes in stress response proteins. Authors - Jeremy A Whitson View Article
human	IHC	Pathol Oncol Res High Expression of Tomm34 and Its Correlations With Clinicopathology in Oral Squamous Cell Carcinoma. Authors - Min Cai View Article
human	WB	J Oral Pathol Med TOMM34 promotes cell proliferation, migration and invasion of OSCC and modulates mitochondrial function Authors - Yunyi Huang KD Validated View Article

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TOMM34 Polyclonal antibody

TOMM34 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 12196-1-AP

WB analysis of HeLa using 12196-1-AP

IP experiment of HeLa using 12196-1-AP

IHC staining of human urothelial carcinoma using 12196-1-AP

IHC staining of human urothelial carcinoma using 12196-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

The proteome of granulovacuolar degeneration and neurofibrillary tangles in Alzheimer's disease.

Proteomic analysis of the glutathione-deficient LEGSKO mouse lens reveals activation of EMT signaling, loss of lens specific markers, and changes in stress response proteins.

High Expression of Tomm34 and Its Correlations With Clinicopathology in Oral Squamous Cell Carcinoma.

TOMM34 promotes cell proliferation, migration and invasion of OSCC and modulates mitochondrial function