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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TOMM40 Monoclonal antibody (66658-1-Ig)

    WB analysis of HeLa using 66658-1-Ig

    WB result of TOMM40 antibody (66658-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOMM40 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using TOMM40 Monoclonal antibody (66658-1-Ig)

    WB analysis using 66658-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Western Blot (WB) analysis of various lysates using TOMM40 Monoclonal antibody (66658-1-Ig)

    WB analysis using 66658-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TOMM40 Monoclonal antibody (66658-1-Ig)

    WB analysis of HEK-293 using 66658-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TOMM40 Monoclonal antibody (66658-1-Ig)

    WB analysis of HepG2 using 66658-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using TOMM40 Monoclonal antibody (66658-1-Ig)

    WB analysis of human brain using 66658-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using TOMM40 Monoclonal antibody (66658-1-Ig)

    WB analysis of pig brain using 66658-1-Ig

    pig brain tissue, rat brain tissue, and mouse brain tissue were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using TOMM40 Monoclonal antibody (66658-1-Ig)

    IHC staining of mouse liver using 66658-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66658-1-Ig (TOMM40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TOMM40 Monoclonal antibody (66658-1-Ig)

    IF Staining of HepG2 using 66658-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66658-1-Ig (TOMM40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TOMM40 Monoclonal antibody (66658-1-Ig)

    IF Staining of HeLa using 66658-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66658-1-Ig (TOMM40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TOMM40 Monoclonal antibody (66658-1-Ig)

    IF Staining of HepG2 using 66658-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66658-1-Ig (TOMM40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  • KD/KO Validated

TOMM40 Monoclonal antibody

TOMM40 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66658-1-Ig
Clone No.5E2C5
Publications(10)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TOM40, 5E2C5, C19orf1, D19S1177E, p38.5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, human brain tissue, pig brain tissue, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, RAW264.7 cells, K-562 cells, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66658-1-Ig targets TOMM40 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TOMM40 fusion protein Ag13065 相同性解析による交差性が予測される生物種
Full Name translocase of outer mitochondrial membrane 40 homolog (yeast)
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC017224
Gene Symbol TOMM40
Gene ID (NCBI) 10452
RRIDAB_2882015
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO96008
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TOMM40 antibody 66658-1-IgDownload protocol
IHC protocol for TOMM40 antibody 66658-1-IgDownload protocol
IF protocol for TOMM40 antibody 66658-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Science

Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex.

Authors - Qiang Wang
View Article
humanWB

Mol Cell

Dynamic Regulation of Mitochondrial Import by the Ubiquitin System.

Authors - Lilian Phu
View Article
mouseWB,IF

Cell Death Differ

Tufm lactylation regulates neuronal apoptosis by modulating mitophagy in traumatic brain injury

Authors - Weiji Weng
View Article
humanWB

Elife

Hepatic conversion of acetyl-CoA to acetate plays crucial roles in energy stress

Authors - Jinyang Wang
View Article
mouseWB

Sci Signal

A genome-wide screen uncovers multiple roles for mitochondrial nucleoside diphosphate kinase D in inflammasome activation.

Authors - Orna Ernst
View Article
ratWB

Apoptosis

Lactate accelerates vascular calcification through NR4A1-regulated mitochondrial fission and BNIP3-related mitophagy.

Authors - Yi Zhu
View Article