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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TOMM5 Polyclonal antibody

TOMM5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25607-1-AP

Synonyms

TOM5, RP11 263I4.1, C9orf105

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using TOMM5 Polyclonal antibody (25607-1-AP)

    WB analysis of mouse testis using 25607-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25607-1-AP (TOMM5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TOMM5 Polyclonal antibody (25607-1-AP)

    WB analysis of HepG2 using 25607-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25607-1-AP (TOMM5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using TOMM5 Polyclonal antibody (25607-1-AP)

    IHC staining of human ovary cancer using 25607-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25607-1-AP (TOMM5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TOMM5 Polyclonal antibody (25607-1-AP)

    IF Staining of HepG2 using 25607-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOMM5 antibody (25607-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidine (red).

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Tested Applications

Positive WB detected inmouse testis tissue, HepG2 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25607-1-AP targets TOMM5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOMM5 fusion protein Ag22275 相同性解析による交差性が予測される生物種
Full Name translocase of outer mitochondrial membrane 5 homolog (yeast)
Calculated molecular weight 51 aa, 6 kDa
Observed molecular weight 6-10 kDa
GenBank accession numberBC034247
Gene symbol TOMM5
Gene ID (NCBI) 401505
RRIDAB_2880157
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOMM5, also named as C9orf105 and TOM5, has three isoforms with MW 6 kDa and 9-10 kDa.

Protocols

Product Specific Protocols
WB protocol for TOMM5 antibody 25607-1-APDownload protocol
IHC protocol for TOMM5 antibody 25607-1-APDownload protocol
IF protocol for TOMM5 antibody 25607-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols