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TOP2A Polyclonal antibody

Name: TOP2A Polyclonal antibody
Brand: Proteintech
SKU: 24641-1-AP

TOP2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 24641-1-AP

Synonyms

TOPII, TOP2, TOP II, EC:5.6.2.2, DNA topoisomerase 2-alpha

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 24641-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24641-1-AP (TOP2A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 24641-1-AP
WB result of TOP2A antibody (24641-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2A transfected HepG2 cells.
WB analysis of HeLa using 24641-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 24641-1-AP (TOP2A Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 24641-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 24641-1-AP (TOP2A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 24641-1-AP
IP result of anti-TOP2A (IP:24641-1-AP, 4ug; Detection:24641-1-AP 1:1000) with HeLa cells lysate 2000ug.
IHC staining of human pancreas cancer using 24641-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24641-1-AP (TOP2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 24641-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24641-1-AP (TOP2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 24641-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24641-1-AP (TOP2A Antibody) at dilution of 1:50.
IHC staining of human breast cancer using 24641-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24641-1-AP (TOP2A Antibody) at dilution of 1:50.
IF Staining of A431 using 24641-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using TOP2A antibody (24641-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 24641-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293T cells, HeLa cells, HepG2 cells, MDA-MB-231 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human pancreas cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 7 publications below
IHC	See 4 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

24641-1-AP targets TOP2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TOP2A fusion protein Ag19551 相同性解析による交差性が予測される生物種
Full Name	topoisomerase (DNA) II alpha 170kDa
Calculated molecular weight	1531 aa, 174 kDa
Observed molecular weight	174 kDa
GenBank accession number	BC140791
Gene symbol	TOP2A
Gene ID (NCBI)	7153
RRID	AB_2879651
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.

Protocols

Product Specific Protocols
WB protocol for TOP2A antibody 24641-1-AP	Download protocol
IHC protocol for TOP2A antibody 24641-1-AP	Download protocol
IF protocol for TOP2A antibody 24641-1-AP	Download protocol
IP protocol for TOP2A antibody 24641-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Front Cell Dev Biol Chemotherapy- and Immune-Related Gene Panel in Prognosis Prediction and Immune Microenvironment of SCLC. Authors - Meng-Yu Chen View Article
human	WB,IF,IP	Mol Biol Cell SPOP is essential for DNA-protein cross-link repair in prostate cancer cells: SPOP-dependent removal of topoisomerase 2A from the topoisomerase 2A-DNA cleavage complex. Authors - Ryuta Watanabe View Article
		Dev Biol Identification of a key role for permeability glycoprotein in enhancing the cellular defense mechanisms of fertilized oocytes. Authors - Jacinta H Martin View Article
human	WB	Chem Biol Interact SZC015, a synthetic oleanolic acid derivative, induces both apoptosis and autophagy in MCF-7 breast cancer cells. Authors - Jingjun Wu View Article
		Oncol Rep Anticancer effects of β-elemene in gastric cancer cells and its potential underlying proteins: A proteomic study. Authors - Jun-Song Liu View Article
human	IHC	J Clin Neurosci High prevalence of developmental venous anomaly in adult patients with midline thalamic diffuse gliomas. Authors - Xingwang Zhou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TOP2A Polyclonal antibody

TOP2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 24641-1-AP

WB analysis of HepG2 using 24641-1-AP

WB analysis of HeLa using 24641-1-AP

WB analysis of HeLa using 24641-1-AP

IP experiment of HeLa using 24641-1-AP

IHC staining of human pancreas cancer using 24641-1-AP

IHC staining of human pancreas cancer using 24641-1-AP

IHC staining of human breast cancer using 24641-1-AP

IHC staining of human breast cancer using 24641-1-AP

IF Staining of A431 using 24641-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Chemotherapy- and Immune-Related Gene Panel in Prognosis Prediction and Immune Microenvironment of SCLC.

SPOP is essential for DNA-protein cross-link repair in prostate cancer cells: SPOP-dependent removal of topoisomerase 2A from the topoisomerase 2A-DNA cleavage complex.

Identification of a key role for permeability glycoprotein in enhancing the cellular defense mechanisms of fertilized oocytes.

SZC015, a synthetic oleanolic acid derivative, induces both apoptosis and autophagy in MCF-7 breast cancer cells.

Anticancer effects of β-elemene in gastric cancer cells and its potential underlying proteins: A proteomic study.

High prevalence of developmental venous anomaly in adult patients with midline thalamic diffuse gliomas.