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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TOP2A Monoclonal antibody (66541-1-Ig)

    WB analysis using 66541-1-Ig (same clone as 66541-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66541-1-Ig (TOP2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66541-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using TOP2A Monoclonal antibody (66541-1-Ig)

    WB analysis of HepG2 using 66541-1-Ig (same clone as 66541-1-PBS)

    WB result of TOP2A antibody (66541-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2A transfected HepG2 cells. This data was developed using the same antibody clone with 66541-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TOP2A Monoclonal antibody (66541-1-Ig)

    IHC staining of human lung cancer using 66541-1-Ig (same clone as 66541-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66541-1-Ig (TOP2A antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66541-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TOP2A Monoclonal antibody (66541-1-Ig)

    IHC staining of human lung cancer using 66541-1-Ig (same clone as 66541-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66541-1-Ig (TOP2A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66541-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TOP2A Monoclonal antibody (66541-1-Ig)

    IF Staining of HepG2 using 66541-1-Ig (same clone as 66541-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66541-1-Ig (TOP2A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66541-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

TOP2A Monoclonal antibody, PBS Only

TOP2A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66541-1-PBS
Clone No.1E12B3

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DNA topoisomerase 2 alpha, TOP2, TOP2A, TP2A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66541-1-PBS targets TOP2A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TOP2A fusion protein Ag19551 相同性解析による交差性が予測される生物種
Full Name topoisomerase (DNA) II alpha 170kDa
Calculated molecular weight 1531 aa, 174 kDa
Observed molecular weight 174 kDa
GenBank accession numberBC140791
Gene Symbol TOP2A
Gene ID (NCBI) 7153
RRIDAB_2881903
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11388
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.