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TOP2A Recombinant antibody

TOP2A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, ELISA
Cat No. 82738-4-RR
Clone No.1J18

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

TOP2, TOP II, EC:5.6.2.2, DNA topoisomerase 2-alpha, DNA topoisomerase 2 alpha

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inK-562 cells, HeLa cells, HEK-293 cells, A431 cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82738-4-RR targets TOP2A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TOP2A fusion protein Ag19551 相同性解析による交差性が予測される生物種
Full Name topoisomerase (DNA) II alpha 170kDa
Calculated molecular weight 1531 aa, 174 kDa
Observed molecular weight~170 kDa
GenBank accession numberBC140791
Gene Symbol TOP2A
Gene ID (NCBI) 7153
RRIDAB_3086521
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11388
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.

Protocols

Product Specific Protocols
WB protocol for TOP2A antibody 82738-4-RRDownload protocol
IF protocol for TOP2A antibody 82738-4-RRDownload protocol
IP protocol for TOP2A antibody 82738-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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