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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TOP2B Recombinant antibody (84049-1-RR)

    WB analysis using 84049-1-RR (same clone as 84049-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84049-1-RR (TOP2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84049-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TOP2B Recombinant antibody (84049-1-RR)

    IF Staining of HeLa using 84049-1-RR (same clone as 84049-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOP2B antibody (84049-1-RR, Clone: 240949F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84049-1-PBS in a different storage buffer formulation.

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TOP2B Recombinant antibody, PBS Only

TOP2B Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84049-1-PBS
Clone No.240949F10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

TOPIIB, top2beta, EC:5.6.2.2, DNA topoisomerase 2-beta, 240949F10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84049-1-PBS targets TOP2B in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name topoisomerase (DNA) II beta 180kDa
Calculated molecular weight 183 kDa
Observed molecular weight180-190 kDa
GenBank accession numberNM_001068
Gene Symbol TOP2B
Gene ID (NCBI) 7155
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ02880
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TOP2B belongs to the type II topoisomerase family. TOP2B control topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2B makes double-strand breaks. It is indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.