Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, rat liver tissue, MCF-7 cells, mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IF | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
21162-1-AP targets TOX2 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TOX2 fusion protein Ag14772 相同性解析による交差性が予測される生物種 |
| Full Name | TOX high mobility group box family member 2 |
| Calculated molecular weight | 488 aa, 52 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC007636 |
| Gene Symbol | TOX2 |
| Gene ID (NCBI) | 84969 |
| RRID | AB_2878821 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96NM4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TOX2 is a transcription factor belonging to the TOX (thymocyte selection-associated HMG box) family that shares a highly conserved high mobility group DNA-binding domain with the other TOX members. A previous study showed that TOX2 is preferentially expressed in human NK cells among various leukocyte populations and is required for in vitro and in vivo human NK cell differentiation from UCB-derived CD341 hematopoietic stem cells (HSCs) (PMID: 25352127).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TOX2 antibody 21162-1-AP | Download protocol |
| IHC protocol for TOX2 antibody 21162-1-AP | Download protocol |
| IP protocol for TOX2 antibody 21162-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Tox2 is required for the maintenance of GC TFH cells and the generation of memory TFH cells.
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Mol Cancer Super-enhancer-driven TOX2 mediates oncogenesis in Natural Killer/T Cell Lymphoma
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