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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TOX2 Polyclonal antibody

TOX2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30474-1-AP

Synonyms

GCX-1, GCX1, C20orf100

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TOX2 Polyclonal antibody (30474-1-AP)

    WB analysis using 30474-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30474-1-AP (TOX2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using TOX2 Polyclonal antibody (30474-1-AP)

    IF Staining of A431 using 30474-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TOX2 antibody (30474-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inA549 cells, mouse liver tissue, rat liver tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30474-1-AP targets TOX2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOX2 fusion protein Ag31226 相同性解析による交差性が予測される生物種
Full Name TOX high mobility group box family member 2
Calculated molecular weight 488 aa, 52 kDa
Observed molecular weight70 kDa
GenBank accession numberBC007636
Gene symbol TOX2
Gene ID (NCBI) 84969
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOX2 is a transcription factor belonging to the TOX (thymocyte selection-associated HMG box) family that shares a highly conserved high mobility group DNA-binding domain with the other TOX members. A previous study showed that TOX2 is preferentially expressed in human NK cells among various leukocyte populations and is required for in vitro and in vivo human NK cell differentiation from UCB-derived CD341 hematopoietic stem cells (HSCs) (PMID: 25352127).

Protocols

Product Specific Protocols
WB protocol for TOX2 antibody 30474-1-APDownload protocol
IF protocol for TOX2 antibody 30474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols