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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TOX2 Recombinant antibody (84304-5-RR)

    WB analysis using 84304-5-RR (same clone as 84304-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84304-5-RR (TOX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84304-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using TOX2 Recombinant antibody (84304-5-RR)

    FC experiment of A549 using 84304-5-RR (same clone as 84304-5-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug TOX2 Recombinant antibody (84304-5-RR, Clone:241645C11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84304-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using TOX2 Recombinant antibody (84304-5-RR)

    FC experiment of MCF-7 using 84304-5-RR (same clone as 84304-5-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug TOX2 Recombinant antibody (84304-5-RR, Clone:241645C11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84304-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84304-5-RR

    Affinity and Kinetic Characterization of 84304-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84304-5-RR against Human TOX2 were performed. The affinity constant is 110 nM.

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TOX2 Recombinant antibody, PBS Only

TOX2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84304-5-PBS
Clone No.241645C11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

GCX-1, GCX1, C20orf100, 241645C11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84304-5-PBS targets TOX2 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TOX2 fusion protein Ag14772 相同性解析による交差性が予測される生物種
Full Name TOX high mobility group box family member 2
Calculated molecular weight 488 aa, 52 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC007636
Gene Symbol TOX2
Gene ID (NCBI) 84969
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96NM4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TOX2 is a transcription factor belonging to the TOX (thymocyte selection-associated HMG box) family that shares a highly conserved high mobility group DNA-binding domain with the other TOX members. A previous study showed that TOX2 is preferentially expressed in human NK cells among various leukocyte populations and is required for in vitro and in vivo human NK cell differentiation from UCB-derived CD341 hematopoietic stem cells (HSCs) (PMID: 25352127).