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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TP53BP1-Specific Recombinant antibody (83809-1-RR)

    WB analysis using 83809-1-RR (same clone as 83809-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83809-1-RR (TP53BP1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83809-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using TP53BP1-Specific Recombinant antibody (83809-1-RR)

    IHC staining of human ovary cancer using 83809-1-RR (same clone as 83809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83809-1-RR (TP53BP1-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83809-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TP53BP1-Specific Recombinant antibody (83809-1-RR)

    IF Staining of HeLa using 83809-1-RR (same clone as 83809-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TP53BP1-Specific antibody (83809-1-RR, Clone: 240638C6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83809-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using TP53BP1-Specific Recombinant antibody (83809-1-RR)

    FC experiment of HeLa using 83809-1-RR (same clone as 83809-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TP53BP1-Specific Recombinant antibody (83809-1-RR, Clone:240638C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83809-1-PBS in a different storage buffer formulation.

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TP53BP1-Specific Recombinant antibody, PBS Only

TP53BP1-Specific Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83809-1-PBS
Clone No.240638C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

TP53BP1, p53-binding protein 1, p53 binding protein 1, p202, FLJ41424

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83809-1-PBS targets TP53BP1-Specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name tumor protein p53 binding protein 1
Calculated molecular weight 214 kDa
Observed molecular weight450 kDa
GenBank accession numberNM_005657
Gene Symbol TP53BP1
Gene ID (NCBI) 7158
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ12888
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TP53BP1, also named as p53BP1, p202, 53BP1 and FLJ41424, may have a role in checkpoint signaling during mitosis. TP53BP1 enhances TP53-mediated transcriptional activation. TP53BP1 plays a role in the response to DNA damage.