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  • Featured Product
  • KD/KO Validated

TP53RK Polyclonal antibody

TP53RK Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26818-1-AP

Synonyms

BUD32, C20orf64, dJ101A2, Nori 2, Nori 2p, p53 related protein kinase, PRPK, TP53 regulating kinase, TP53RK

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using TP53RK Polyclonal antibody (26818-1-AP)

    WB analysis of Jurkat using 26818-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26818-1-AP (TP53RK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TP53RK Polyclonal antibody (26818-1-AP)

    WB analysis of HepG2 using 26818-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26818-1-AP (TP53RK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using TP53RK Polyclonal antibody (26818-1-AP)

    IP experiment of Jurkat using 26818-1-AP

    IP result of anti-TP53RK (IP:26818-1-AP, 4ug; Detection:26818-1-AP 1:700) with Jurkat cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using TP53RK Polyclonal antibody (26818-1-AP)

    IHC staining of human testis using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using TP53RK Polyclonal antibody (26818-1-AP)

    IHC staining of human testis using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, HepG2 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26818-1-AP is TP53RK Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TP53RK fusion protein Ag25326 相同性解析による交差性が予測される生物種
Full Name TP53 regulating kinase
Calculated molecular weight 28 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC009727
Gene symbol TP53RK
Gene ID (NCBI) 112858
RRIDAB_2880647
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TP53RK antibody 26818-1-APDownload protocol
IHC protocol for TP53RK antibody 26818-1-APDownload protocol
IP protocol for TP53RK antibody 26818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Biol Med

Systematic screening for potential therapeutic targets in osteosarcoma through a kinome-wide CRISPR-Cas9 library.

Authors - Yuanzhong Wu
  • KO Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Evangelia (Verified Customer) (10-05-2022)

It could be used in higher dilution. The 1/1000 works really strong.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: -