Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells, MCF-7 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
29394-1-AP targets TPBG in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.
| Tested Reactivity | Human, rat, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TPBG fusion protein Ag30019 相同性解析による交差性が予測される生物種 |
| Full Name | trophoblast glycoprotein |
| Calculated molecular weight | 46 kDa |
| Observed molecular weight | 72-80 kDa |
| GenBank accession number | NM_006670 |
| Gene Symbol | TPBG |
| Gene ID (NCBI) | 7162 |
| RRID | AB_2918290 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13641 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
Trophoblast glycoprotein (TPBG), also termed 5T4, is a 72 kDa transmembrane glycoprotein and an extensively investigated oncofetal antigen, which is limited in normal adult tissues, but highly expressed in various types of cancer in human. TPBG is a novel PKCa-dependent phosphoprotein in retinal rod bipolar cells (RBCs). (PMID: 18097562, PMID: 31255707)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TPBG antibody 29394-1-AP | Download protocol |
| IHC protocol for TPBG antibody 29394-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Manag Res MIR31HG Expression Predicts Poor Prognosis and Promotes Colorectal Cancer Progression. |