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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TPBG Polyclonal antibody

TPBG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29394-1-AP

Synonyms

5T4, 5T4 AG, 5T4 oncofetal antigen, M6P1, TPBG, trophoblast glycoprotein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPBG Polyclonal antibody (29394-1-AP)

    WB analysis using 29394-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29394-1-AP (TPBG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using TPBG Polyclonal antibody (29394-1-AP)

    IHC staining of mouse eye using 29394-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 29394-1-AP (TPBG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using TPBG Polyclonal antibody (29394-1-AP)

    IHC staining of mouse eye using 29394-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 29394-1-AP (TPBG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293T cells, MCF-7 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

29394-1-AP targets TPBG in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPBG fusion protein Ag30019 相同性解析による交差性が予測される生物種
Full Name trophoblast glycoprotein
Calculated molecular weight 46 kDa
Observed molecular weight72-80 kDa
GenBank accession numberNM_006670
Gene symbol TPBG
Gene ID (NCBI) 7162
RRIDAB_2918290
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Trophoblast glycoprotein (TPBG), also termed 5T4, is a 72 kDa transmembrane glycoprotein and an extensively investigated oncofetal antigen, which is limited in normal adult tissues, but highly expressed in various types of cancer in human. TPBG is a novel PKCa-dependent phosphoprotein in retinal rod bipolar cells (RBCs). (PMID: 18097562, PMID: 31255707)

Protocols

Product Specific Protocols
WB protocol for TPBG antibody 29394-1-APDownload protocol
IHC protocol for TPBG antibody 29394-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancer Manag Res

MIR31HG Expression Predicts Poor Prognosis and Promotes Colorectal Cancer Progression.

Authors - Jianlong Wang
View Article