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  • Featured Product
  • KD/KO Validated

TPC1 Polyclonal antibody, PBS Only

TPC1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 23758-1-PBS

Synonyms

TPCN1, Voltage-dependent calcium channel protein TPC1, two pore segment channel 1, Two pore channel protein 1, KIAA1169

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPC1 Polyclonal antibody (23758-1-AP)

    WB analysis using 23758-1-AP (same clone as 23758-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23758-1-AP (TPC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23758-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using TPC1 Polyclonal antibody (23758-1-AP)

    IHC staining of mouse heart using 23758-1-AP (same clone as 23758-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 23758-1-AP (TPCN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23758-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using TPC1 Polyclonal antibody (23758-1-AP)

    IHC staining of mouse heart using 23758-1-AP (same clone as 23758-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 23758-1-AP (TPCN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23758-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TPC1 Polyclonal antibody (23758-1-AP)

    IF Staining of HepG2 using 23758-1-AP (same clone as 23758-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TPC1 antibody (23758-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 23758-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23758-1-PBS targets TPC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPC1 fusion protein Ag20607 相同性解析による交差性が予測される生物種
Full Name two pore segment channel 1
Calculated molecular weight 816 aa, 94 kDa
Observed molecular weight 94 kDa
GenBank accession numberBC136795
Gene symbol TPCN1
Gene ID (NCBI) 53373
RRIDAB_2879317
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Two-pore channels (TPCs) are ancient members of the voltage-gated ion channel superfamily, are thought to be non-selective cation channels permeable to Ca2+. They mediate their physiological effects through releasing Ca2+ from acidic organelles in response to cues such as the second messenger, NAADP (nicotinic acid adenine dinucleotide phosphate). Genetic knockout and pharmacological inhibition experiments demonstrate that the two-pore channels, TPC1 and TPC2, are required for infection by Filoviruses Ebola and Marburg in mice.(PMID: 29746898; PMID 25722412) Two pore segment channel 1 (TPC1) is a human protein encoded by the TPCN1 gene.