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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TPD52 Polyclonal antibody

TPD52 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27648-1-AP

Synonyms

D52, hD52, N8L, PC 1, PrLZ, Protein N8, TPD52, tumor protein D52

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPD52 Polyclonal antibody (27648-1-AP)

    WB analysis using 27648-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27648-1-AP (TPD52 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TPD52 Polyclonal antibody (27648-1-AP)

    WB analysis using 27648-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27648-1-AP (TPD52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TPD52 Polyclonal antibody (27648-1-AP)

    IHC staining of human stomach cancer using 27648-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27648-1-AP (TPD52 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TPD52 Polyclonal antibody (27648-1-AP)

    IHC staining of human stomach cancer using 27648-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27648-1-AP (TPD52 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inL02 cells, HepG2 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27648-1-AP targets TPD52 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPD52 fusion protein Ag26503 相同性解析による交差性が予測される生物種
Full Name tumor protein D52
Calculated molecular weight 24 kDa
Observed molecular weight 23-27 kDa
GenBank accession numberBC018117
Gene symbol TPD52
Gene ID (NCBI) 7163
RRIDAB_3085979
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TPD52 antibody 27648-1-APDownload protocol
IHC protocol for TPD52 antibody 27648-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols