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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TPD52L2 Monoclonal antibody

TPD52L2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3B3C6

Cat no : 68077-1-Ig

Synonyms

D54, DKFZp686A1765, hD54, TPD52L2, TPD54, tumor protein D52 like 2, Tumor protein D54

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPD52L2 Monoclonal antibody (68077-1-Ig)

    WB analysis using 68077-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68077-1-Ig (TPD52L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCI-H1299 cells using TPD52L2 Monoclonal antibody (68077-1-Ig)

    WB analysis of NCI-H1299 using 68077-1-Ig

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 68077-1-Ig (TPD52L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TPD52L2 Monoclonal antibody (68077-1-Ig)

    WB analysis of MCF-7 using 68077-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 68077-1-Ig (TPD52L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using TPD52L2 Monoclonal antibody (68077-1-Ig)

    WB analysis of Neuro-2a using 68077-1-Ig

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 68077-1-Ig (TPD52L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using TPD52L2 Monoclonal antibody (68077-1-Ig)

    WB analysis of C6 using 68077-1-Ig

    C6 cells were subjected to SDS PAGE followed by western blot with 68077-1-Ig (TPD52L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TPD52L2 Monoclonal antibody (68077-1-Ig)

    IHC staining of human breast cancer using 68077-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68077-1-Ig (TPD52L2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TPD52L2 Monoclonal antibody (68077-1-Ig)

    IHC staining of human breast cancer using 68077-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68077-1-Ig (TPD52L2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using TPD52L2 Monoclonal antibody (68077-1-Ig)

    IF Staining of HCT 116 using 68077-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HCT 116 cells using TPD52L2 antibody (68077-1-Ig, Clone: 3B3C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inCalu-3 cells, NCI-H1299 cells, MCF-7 cells, C6 cells, Neuro-2a cells, HCT 116 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHCT 116 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68077-1-Ig targets TPD52L2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen fusion protein 相同性解析による交差性が予測される生物種
Full Name tumor protein D52-like 2
Calculated molecular weight 206 aa, 22 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC006804
Gene symbol TPD52L2
Gene ID (NCBI) 7165
RRIDAB_2918815
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tumor protein D52-like proteins (TPD52) are small coiled-coil motif bearing proteins that were first identified in breast carcinoma. Three human TPD52 members had been identified, named hD52 (TPD52), hD53 (TPD52L1), and hD54 (TPD52L2). The most important characteristic of the protein family is a highly conserved coiled-coil motif that is required for homo- and heteromeric interaction with other TPD52-like proteins. TPD52 and related proteins have been implicated in cell proliferation, apoptosis, and vesicle trafficking. TPD52L2 has five isoforms produced by alternative splicing, and its multiple sites have been identified to be phosphorylated. Interaction of TPD52L2 with MAL2, a novel member of the MAL proteolipid family, may be required for the role of TPD52L2 in vesicle transport.

Protocols

Product Specific Protocols
WB protocol for TPD52L2 antibody 68077-1-IgDownload protocol
IHC protocol for TPD52L2 antibody 68077-1-IgDownload protocol
IF protocol for TPD52L2 antibody 68077-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols