Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat brain tissue |
| Positive IHC detected in | human liver cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
22590-1-AP targets TPH2 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TPH2 fusion protein Ag18408 相同性解析による交差性が予測される生物種 |
| Full Name | tryptophan hydroxylase 2 |
| Calculated molecular weight | 490 aa, 56 kDa |
| Observed molecular weight | 56 kDa |
| GenBank accession number | BC114442 |
| Gene Symbol | TPH2 |
| Gene ID (NCBI) | 121278 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IWU9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Publications
| Species | Application | Title |
|---|---|---|
Neurosci Bull Genetic Architecture and Functional Implications of the CSF-Contacting Nucleus |