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TPI1 Polyclonal antibody

Name: TPI1 Polyclonal antibody
Brand: Proteintech
SKU: 10713-1-AP

TPI1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10713-1-AP

Synonyms

Triose-phosphate isomerase, TPI, TIM, Methylglyoxal synthase, EC:5.3.1.1

Validation Data Gallery

Filter:

AllWBIP

WB analysis using 10713-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10713-1-AP
WB result of TPI1 antibody (10713-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPI1 transfected HEK-293 cells.
WB analysis of rat kidney using 10713-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of RAW 264.7 using 10713-1-AP
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 10713-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 10713-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10713-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 10713-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10713-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 10713-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 10713-1-AP
IP result of anti-TPI1 (IP:10713-1-AP, 3ug; Detection:10713-1-AP 1:1000) with HEK-293 cells lysate 2500ug.

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Cat No. 10713-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, A431 cells, HepG2 cells, rat liver tissue, rat kidney tissue, RAW 264.7 cells, mouse liver tissue, mouse kidney tissue, NIH/3T3 cells
Positive IP detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 34 publications below
IHC	See 4 publications below
IF	See 3 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

10713-1-AP targets TPI1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken, yeast, drosophila
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TPI1 fusion protein Ag1175 相同性解析による交差性が予測される生物種
Full Name	triosephosphate isomerase 1
Calculated molecular weight	27 kDa
Observed molecular weight	27 kDa
GenBank accession number	BC007086
Gene symbol	TPI1
Gene ID (NCBI)	7167
RRID	AB_2207716
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TPI1(Triosephosphate isomerase) is also named as TIM, TPI and belongs to the triosephosphate isomerase family. It is a key enzyme in cell metabolism that controls the glycolytic flow and energy production through the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (G3P)(PMID:19251756). Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). It can exsit as a homodimer(PMID:18562316).

Protocols

Product Specific Protocols
WB protocol for TPI1 antibody 10713-1-AP	Download protocol
IP protocol for TPI1 antibody 10713-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell The histone deacetylase Sirt6 regulates glucose homeostasis via Hif1alpha. Authors - Zhong Lei L View Article
		Nat Chem Biol SF2312 is a natural phosphonate inhibitor of enolase. Authors - Paul G Leonard View Article
human	WB	Nat Commun Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk. Authors - Xiao-Hui Wang View Article
human	WB	Cell Res cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. Authors - Linchong Sun View Article
human	WB	Nat Commun Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. Authors - Xiaoyu Ma View Article
human,mouse	WB,IHC,IF	Brain Amyloid-dependent triosephosphate isomerase nitrotyrosination induces glycation and tau fibrillation. Authors - Guix Francesc X FX View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TPI1 Polyclonal antibody

TPI1 Polyclonal Antibody for WB, IP, ELISA

Validation Data Gallery

WB analysis using 10713-1-AP

WB analysis of HEK-293 using 10713-1-AP

WB analysis of rat kidney using 10713-1-AP

WB analysis of RAW 264.7 using 10713-1-AP

WB analysis of mouse liver using 10713-1-AP

WB analysis of A431 using 10713-1-AP

WB analysis of HepG2 using 10713-1-AP

WB analysis of rat liver using 10713-1-AP

WB analysis of HEK-293 using 10713-1-AP

WB analysis of mouse kidney using 10713-1-AP

IP experiment of HEK-293 using 10713-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The histone deacetylase Sirt6 regulates glucose homeostasis via Hif1alpha.

SF2312 is a natural phosphonate inhibitor of enolase.

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.

Amyloid-dependent triosephosphate isomerase nitrotyrosination induces glycation and tau fibrillation.