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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using TPI1 Monoclonal antibody (60036-2-Ig)

    WB analysis of HEK-293 using 60036-2-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60036-2-Ig (TPI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TPI1 Monoclonal antibody (60036-2-Ig)

    WB analysis of HeLa using 60036-2-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60036-2-Ig (TPI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TPI1 Monoclonal antibody (60036-2-Ig)

    WB analysis of HeLa using 60036-2-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60036-2-Ig (TPI1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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TPI1 Monoclonal antibody

TPI1 Monoclonal Antibody for WB, ELISA
Cat No. 60036-2-Ig
Clone No.3G11G3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

TIM, TPI, TPI1, Triose phosphate isomerase, Triosephosphate isomerase, triosephosphate isomerase 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60036-2-Ig targets TPI1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TPI1 fusion protein Ag1175 相同性解析による交差性が予測される生物種
Full Name triosephosphate isomerase 1
Calculated molecular weight 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC007086
Gene Symbol TPI1
Gene ID (NCBI) 7167
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP60174
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TPI1(Triosephosphate isomerase) is also named as TIM, TPI and belongs to the triosephosphate isomerase family. It is a key enzyme in cell metabolism that controls the glycolytic flow and energy production through the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (G3P)(PMID:19251756). Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). It can exsit as a homodimer(PMID:18562316).