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TPI1 Monoclonal antibody
TPI1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2E7E5
Cat no : 67515-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
67515-1-Ig targets TPI1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TPI1 fusion protein Ag16653 相同性解析による交差性が予測される生物種 |
| Full Name | triosephosphate isomerase 1 |
| Calculated molecular weight | 27 kDa |
| Observed molecular weight | 27 kDa |
| GenBank accession number | BC007086 |
| Gene symbol | TPI1 |
| Gene ID (NCBI) | 7167 |
| RRID | AB_2882736 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TPI1(Triosephosphate isomerase) is also named as TIM, TPI and belongs to the triosephosphate isomerase family. It is a key enzyme in cell metabolism that controls the glycolytic flow and energy production through the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (G3P)(PMID:19251756). Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). It can exsit as a homodimer(PMID:18562316).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TPI1 antibody 67515-1-Ig | Download protocol |
| IHC protocol for TPI1 antibody 67515-1-Ig | Download protocol |
| IF protocol for TPI1 antibody 67515-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Food Chem Phosphorylation and acetylation responses of glycolytic enzymes in meat to different chilling rates | ||
Int Immunopharmacol Candida tropicalis induces NLRP3 inflammasome activation via glycogen metabolism-dependent glycolysis and JAK-STAT1 signaling pathway in myeloid-derived suppressor cells to promote colorectal carcinogenesis |
