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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TPM1 Polyclonal antibody, PBS Only

TPM1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28477-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPM1 Polyclonal antibody (28477-1-AP)

    WB analysis using 28477-1-AP (same clone as 28477-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28477-1-AP (TPM1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28477-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TPM1 Polyclonal antibody (28477-1-AP)

    IHC staining of human stomach cancer using 28477-1-AP (same clone as 28477-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28477-1-AP (TPM1 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28477-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using TPM1 Polyclonal antibody (28477-1-AP)

    IHC staining of human colon using 28477-1-AP (same clone as 28477-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28477-1-AP (TPM1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28477-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using TPM1 Polyclonal antibody (28477-1-AP)

    IHC staining of mouse skeletal muscle using 28477-1-AP (same clone as 28477-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28477-1-AP (TPM1 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28477-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28477-1-PBS targets TPM1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPM1 fusion protein Ag29525 相同性解析による交差性が予測される生物種
Full Name tropomyosin 1 (alpha)
Observed molecular weight 30 kDa
GenBank accession numberBC053545
Gene symbol TPM1
Gene ID (NCBI) 7168
RRIDAB_2881151
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.