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  • Featured Product
  • KD/KO Validated

TPM2 Polyclonal antibody

TPM2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55443-1-AP

Synonyms

AMCD1, Beta tropomyosin, DA1, DA2B, TMSB, TPM2, Tropomyosin 2, tropomyosin 2 (beta), Tropomyosin beta chain

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using TPM2 Polyclonal antibody (55443-1-AP)

    WB analysis of mouse heart using 55443-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 55443-1-AP (TPM2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TPM2 Polyclonal antibody (55443-1-AP)

    WB analysis of HeLa using 55443-1-AP

    WB result of TPM2 antibody (55443-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPM2 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TPM2 Polyclonal antibody (55443-1-AP)

    IHC staining of human breast cancer using 55443-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55443-1-AP (TPM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TPM2 Polyclonal antibody (55443-1-AP)

    IHC staining of human breast cancer using 55443-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55443-1-AP (TPM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TPM2 Polyclonal antibody (55443-1-AP)

    IHC staining of human breast cancer using 55443-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55443-1-AP (TPM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TPM2 Polyclonal antibody (55443-1-AP)

    IHC staining of human breast cancer using 55443-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55443-1-AP (TPM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse heart tissue, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55443-1-AP targets TPM2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name tropomyosin 2 (beta)
Calculated molecular weight 33 kDa
Observed molecular weight 37 kDa
GenBank accession numberNM_003289
Gene symbol TPM2
Gene ID (NCBI) 7169
RRIDAB_2881335
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TPM2 antibody 55443-1-APDownload protocol
IHC protocol for TPM2 antibody 55443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols