Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of mouse skeletal muscle using 10737-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10737-1-AP
WB result of TPM3 antibody (10737-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPM3 transfected HepG2 cells.
WB analysis using 10737-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 10737-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 10737-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 10737-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 10737-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10737-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10737-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10737-1-AP
IP result of anti-TPM3 (IP:10737-1-AP, 4ug; Detection:10737-1-AP 1:300) with HeLa cells lysate 2000ug.
IHC staining of human colon cancer using 10737-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10737-1-AP (TPM3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

TPM3 Polyclonal antibody

TPM3 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10737-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Tropomyosin-5, Tropomyosin-3, Tropomyosin alpha-3 chain, Tropomyosin alpha 3 chain, Gamma-tropomyosin

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (12)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse skeletal muscle tissue, mouse heart tissue, human heart tissue, HeLa cells, HEK-293 cells, rat heart tissue, HepG2 cells, rat skeletal muscle tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 10 publications below
IHC	See 2 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

10737-1-AP targets TPM3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TPM3 fusion protein Ag1171 相同性解析による交差性が予測される生物種
Full Name	tropomyosin 3
Calculated molecular weight	33 kDa
Observed molecular weight	38-40 kDa
GenBank accession number	BC008407
Gene Symbol	TPM3
Gene ID (NCBI)	7170
RRID	AB_2205671
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P06753
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Tropomyosins are ubiquitous proteins of 35 to 45 kDa associated with the actin filaments of myofibrils and stress fibers. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins.TPM2 subunit constitutes one part of the TPM dimer, and TPM1 or TPM3 constitutes the other part of the TPM dimer (22064019), causing an extra band of ~70kDa.

Protocols

Product Specific Protocols
WB protocol for TPM3 antibody 10737-1-AP	Download protocol
IHC protocol for TPM3 antibody 10737-1-AP	Download protocol
IP protocol for TPM3 antibody 10737-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Bone Res Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma Authors - Ming Luo View Article
	IP	Nucleic Acids Res Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing. Authors - Ken Asada View Article
human	WB	Oncogene MTBP suppresses cell migration and filopodia formation by inhibiting ACTN4. Authors - Agarwal N N View Article
human,mouse	WB	Oncol Res LncRNA WEE2-AS1 Knockdown Inhibits the Proliferation, Migration and 3 Invasion of Glioma Cells via Regulating miR-29b-2-5p/TPM3 Axis. Authors - Zhen Jia View Article
	WB	J Proteome Res Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy Authors - Yuan Lin View Article
human	IHC	Bioengineered PolyC-RNA-binding protein 1 (PCBP1) enhances tropomyosin 3 (TPM3) mRNA stability to promote the progression of esophageal squamous cell carcinoma. Authors - Kaiming Peng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse skeletal muscle using 10737-1-AP

WB analysis of HepG2 using 10737-1-AP

WB analysis using 10737-1-AP

WB analysis of mouse heart using 10737-1-AP

WB analysis of mouse skeletal muscle using 10737-1-AP

WB analysis of human heart using 10737-1-AP

WB analysis of rat heart using 10737-1-AP

WB analysis of HeLa using 10737-1-AP

WB analysis of HEK-293 using 10737-1-AP

IP experiment of HeLa using 10737-1-AP

IHC staining of human colon cancer using 10737-1-AP

TPM3 Polyclonal antibody

TPM3 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma

Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing.

MTBP suppresses cell migration and filopodia formation by inhibiting ACTN4.

LncRNA WEE2-AS1 Knockdown Inhibits the Proliferation, Migration and 3 Invasion of Glioma Cells via Regulating miR-29b-2-5p/TPM3 Axis.

Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy

PolyC-RNA-binding protein 1 (PCBP1) enhances tropomyosin 3 (TPM3) mRNA stability to promote the progression of esophageal squamous cell carcinoma.