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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TPM3 Polyclonal antibody

TPM3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55444-1-AP

Synonyms

Gamma tropomyosin, hscp30, hTM5, NEM1, OK/SW cl.5, TM 5, TM3, TM30, TM30nm, TPM3, TPMsk3, TRK, tropomyosin 3, Tropomyosin 5, Tropomyosin alpha 3 chain

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using TPM3 Polyclonal antibody (55444-1-AP)

    WB analysis of mouse skeletal muscle using 55444-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55444-1-AP (TPM3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TPM3 Polyclonal antibody (55444-1-AP)

    WB analysis of HepG2 using 55444-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55444-1-AP (TPM3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using TPM3 Polyclonal antibody (55444-1-AP)

    IHC staining of human colon using 55444-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55444-1-AP (TPM3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using TPM3 Polyclonal antibody (55444-1-AP)

    IHC staining of human colon using 55444-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55444-1-AP (TPM3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TPM3 Polyclonal antibody (55444-1-AP)

    IHC staining of human colon cancer using 55444-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55444-1-AP (TPM3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TPM3 Polyclonal antibody (55444-1-AP)

    IHC staining of human colon cancer using 55444-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55444-1-AP (TPM3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TPM3 Polyclonal antibody (55444-1-AP)

    IHC staining of human liver cancer using 55444-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55444-1-AP (TPM3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TPM3 Polyclonal antibody (55444-1-AP)

    IHC staining of human liver cancer using 55444-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55444-1-AP (TPM3 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue, HepG2 cells
Positive IHC detected inhuman colon tissue, human colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55444-1-AP targets TPM3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name tropomyosin 3
Calculated molecular weight 33 kDa
Observed molecular weight 40-42 kDa
GenBank accession numberNM_153649
Gene symbol TPM3
Gene ID (NCBI) 7170
RRIDAB_2881336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TPM3 antibody 55444-1-APDownload protocol
IHC protocol for TPM3 antibody 55444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols